PURIFICATION AND PROPERTIES OF PYRUVATE-KINASE FROM THERMOPLASMA-ACIDOPHILUM

被引：15
作者：
POTTER, S
FOTHERGILLGILMORE, LA
机构：
来源：
FEMS MICROBIOLOGY LETTERS | 1992年 / 94卷 / 03期
关键词：
PYRUVATE KINASE; ARCHAEBACTERIA; ENZYME PURIFICATION; AMINO ACID SEQUENCE; THERMOPLASMA-ACIDOPHILUM;
D O I：
10.1111/j.1574-6968.1992.tb05324.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Thermoplasma acidophilum is a thermoacidophilic archaebacterium occupying a paradoxical place in phylogenetic trees (phenotypically it is a thermoacidophile but phylogenetically it classifies with the methanogens). To better understand its phylogeny, the pyruvate kinase from this organism is being investigated as a molecular marker. The enzyme has been purified and has a native M(r) of 250000. It consists of four, apparently identical subunits each of M(r) 60 000. No remarkable kinetic differences have been found between this thermophilic enzyme and its mesophilic counterparts other than its greater thermostability. Its amino acid composition has been determined and some partial sequencing as been done.
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页码：235 / 239
页数：5
相关论文
共 16 条
[1]  
BRANDEN C, 1991, INTRO PROTEIN STRUCT, P259
[2]  
BRIGHT J, 1992, IN PRESS EUR J BIOCH
[3]   PYRUVATE KINASE FROM MUSCLE [J].
BUCHER, T ;
PFLEIDERER, G .
METHODS IN ENZYMOLOGY, 1955, 1 :435-440
[4]  
Danson M J, 1988, Adv Microb Physiol, V29, P165, DOI 10.1016/S0065-2911(08)60348-3
[5]   CLONING AND SEQUENCING THE GENE ENCODING 3-PHOSPHOGLYCERATE KINASE FROM MESOPHILIC METHANOBACTERIUM-BRYANTII AND THERMOPHILIC METHANOTHERMUS-FERVIDUS [J].
FABRY, S ;
HEPPNER, P ;
DIETMAIER, W ;
HENSEL, R .
GENE, 1990, 91 (01) :19-25
[6]   PHYLOGENY CONGRUENCE ANALYSIS AND ISOZYME CLASSIFICATION - THE PYRUVATE-KINASE SYSTEM [J].
GUDERLEY, H ;
FOURNIER, P ;
AUCLAIR, JC .
JOURNAL OF THEORETICAL BIOLOGY, 1989, 140 (02) :205-220
[7]   EVIDENCE THAT GLUTATHIONE S-TRANSFERASE-B1B1 AND S-TRANSFERASE-B2B2 ARE THE PRODUCTS OF SEPARATE GENES AND THAT THEIR EXPRESSION IN HUMAN-LIVER IS SUBJECT TO INTER-INDIVIDUAL VARIATION - MOLECULAR RELATIONSHIPS BETWEEN THE B1-SUBUNIT AND B2-SUBUNITS AND OTHER ALPHA-CLASS GLUTATHIONE S-TRANSFERASE [J].
HAYES, JD ;
KERR, LA ;
CRONSHAW, AD .
BIOCHEMICAL JOURNAL, 1989, 264 (02) :437-445
[8]   SEQUENCE COMPARISON OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES FROM THE 3 URKINGDOMS - EVOLUTIONARY IMPLICATION [J].
HENSEL, R ;
ZWICKL, P ;
FABRY, S ;
LANG, J ;
PALM, P .
CANADIAN JOURNAL OF MICROBIOLOGY, 1989, 35 (01) :81-85
[9]   ORIGIN OF THE EUKARYOTIC NUCLEUS DETERMINED BY RATE-INVARIANT ANALYSIS OF RIBOSOMAL-RNA SEQUENCES [J].
LAKE, JA .
NATURE, 1988, 331 (6152) :184-186
[10]   THE STRUCTURE OF CAT MUSCLE PYRUVATE-KINASE [J].
MUIRHEAD, H ;
CLAYDEN, DA ;
BARFORD, D ;
LORIMER, CG ;
FOTHERGILLGILMORE, LA ;
SCHILTZ, E ;
SCHMITT, W .
EMBO JOURNAL, 1986, 5 (03) :475-481
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