RAPD and SCAR Markers Linked to Tea-Mosquito Resistance in Tea

被引：4
|
作者：
Suganthi, M. [1 ]
Senthilkumar, P. [1 ,3 ]
Arvinth, S. [1 ,2 ]
Rajkumar, R. [1 ]
Chandrashekara, K. N. [1 ]
机构：
[1] UPASI Tea Res Inst, Plant Physiol & Biotechnol Div, Coimbatore 642127, Tamil Nadu, India
[2] Vanavarayar Inst Agr, Dept Biotechnol, Pollachi, India
[3] SRM Univ, Dept Genet Engn, Kattankulathur, India
来源：
JOURNAL OF CROP IMPROVEMENT | 2014年 / 28卷 / 06期
关键词：
molecular markers; Camellia spp; tea mosquito; tea cultivars; germplasm;
D O I：
10.1080/15427528.2014.943355
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Tea mosquito (Helopeltis theivora) is a serious pest in tea (Camellia sinensis) growing regions, causing severe crop loss. Identification of markers associated with tea-mosquito resistance is necessary to develop cultivars tolerant to tea mosquito from the available germplasm. This study was conducted to identify random amplified polymorphic DNA (RAPD) markers associated with tolerance against tea mosquito. The RAPD markers were then sequenced and converted into sequence-characterized amplified region (SCAR) markers. A total of 138 decamer primers were used to screen genomic DNA from four susceptible and four relatively tolerant tea cultivars. Only three RAPD markers (OPW-03(725), OPT-01(625), and OPG-11(750)) were linked to tea-mosquito resistance. These markers were converted into co-dominant SCAR markers, Rht1, Rht2, and Rht3. This is the first study reporting the development of markers for tea-mosquito resistance. These markers can be used as an important tool to screen tea cultivars for tea-mosquito resistance/susceptibility.
引用
收藏
页码：795 / 803
页数：9
相关论文
共 50 条
  • [41] Identification and validation of RAPD and SCAR markers linked to the gene Er3 conferring resistance to Erysiphe pisi DC in pea
    Fondevilla, S.
    Rubiales, D.
    Moreno, M. T.
    Torres, A. M.
    MOLECULAR BREEDING, 2008, 22 (02) : 193 - 200
  • [42] Development of RAPD and SCAR markers linked to the Pvr4 locus for resistance to PVY in pepper (Capsicum annuum L.)
    M. Arnedo-Andrés
    R. Gil-Ortega
    M. Luis-Arteaga
    J. Hormaza
    Theoretical and Applied Genetics, 2002, 105 : 1067 - 1074
  • [43] Identification of RAPD and SCAR markers linked to northern leaf blight resistance in waxy corn (Zea mays var. ceratina)
    Juthaporn Khampila
    Kamol Lertrat
    Weerasak Saksirirat
    Jirawat Sanitchon
    Nooduan Muangsan
    Piyada Theerakulpisut
    Euphytica, 2008, 164 : 615 - 625
  • [44] Identification of RAPD and SCAR markers linked to northern leaf blight resistance in waxy corn (Zea mays var. ceratina)
    Khampila, Juthaporn
    Lertrat, Kamol
    Saksirirat, Weerasak
    Sanitchon, Jirawat
    Muangsan, Nooduan
    Theerakulpisut, Piyada
    EUPHYTICA, 2008, 164 (03) : 615 - 625
  • [45] Identification of RAPD, SCAR, and RFLP markers tightly linked to nematode resistance genes introgressed from Arachis cardenasii into Arachis hypogaea
    Garcia, GM
    Stalker, HT
    Shroeder, E
    Kochert, G
    GENOME, 1996, 39 (05) : 836 - 845
  • [46] Assessment of genetic diversity of Tea (Camellia sinensis LO Kuntze) as revealed by RAPD - PCR markers
    Goonetillieke, W. A. S. N. S. T.
    Priyantha, P. G. C.
    Mewan, K. M.
    Gunasekare, M. T. K.
    JOURNAL OF THE NATIONAL SCIENCE FOUNDATION OF SRI LANKA, 2009, 37 (02): : 147 - 150
  • [47] Detection of intra-clonal genetic variability in vegetatively propagated tea using RAPD markers
    Singh, M
    Saroop, J
    Dhiman, B
    BIOLOGIA PLANTARUM, 2004, 48 (01) : 113 - 115
  • [48] Development of resistance to endosulphan in populations of the tea mosquito bug Helopeltis theivora (Heteroptera: Miridae) from organic and conventional tea plantations in India
    Roy S.
    Mukhopadhyay A.
    Gurusubramanian G.
    International Journal of Tropical Insect Science, 2010, 30 (2) : 61 - 66
  • [49] Evaluation of sex specific RAPD and SCAR markers linked to papaya (Carica papaya L.)
    Modi, Arpan
    Suthar, Kiran
    Thakkar, Puja
    Mankad, Mounil C.
    Kumari, Swati
    Narayanan, Subhash
    Singh, Amritpal S.
    Kumar, Nitish
    BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2018, 16 : 271 - 276
  • [50] Identification of RAPD markers linked to eastern filbert blight resistance in hazelnut
    Davis, JW
    Mehlenbacher, SA
    FOURTH INTERNATIONAL SYMPOSIUM ON HAZELNUT, 1997, (445): : 553 - 556
12345