IDENTIFICATION OF A CELL-SURFACE COMPONENT OF SWISS 3T3 CELLS ASSOCIATED WITH AN INHIBITION OF CELL-DIVISION

被引：3

作者：

LAKSHMANARAO, SS ^{[1
]}

TOOLESIMMS, WE ^{[1
]}

FATTAEY, HK ^{[1
]}

LEACH, RJ ^{[1
]}

JOHNSON, TC ^{[1
]}

机构：

[1] KANSAS STATE UNIV AGR & APPL SCI,CTR BASIC CANC RES & BIOSERVE SPACE TECHNOL,DIV BIOL,MANHATTAN,KS 66506

来源：

EXPERIMENTAL CELL RESEARCH | 1991年 / 195卷 / 02期

基金：

美国国家航空航天局;

关键词：

D O I：

10.1016/0014-4827(91)90391-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Polyclonal IgG, prepared to a purified bovine cell surface sialoglycopeptide (SGP) inhibitor of cell division, was used to identify an antigenically related molecule on the surface of Swiss 3T3 cells. SDS-PAGE and Western analyses showed that the anti-SGP antibody was monospecific and primarily recognized a 66-kDa protein of 3T3 cell membranes. Treatment of intact 3T3 cells or 3T3 cell membranes with either broad and phosphatidylinositol-specific phospholipase C enzymes suggested that the antigenic material most likely existed as an integral membrane molecule, or associated as a multimeric complex, and was not anchored at the cell surface by a phospholipid. The addition of anti-SGP IgG to 3T3 cell monolayer cultures was shown to promote cell division, suggesting a regulatory function for the membrane-associated molecule. © 1991.

引用

页码：412 / 415

页数：4