PYRUVATE-FORMATE-LYASE-DEACTIVASE AND ACETYL-COA REDUCTASE ACTIVITIES OF ESCHERICHIA-COLI RESIDE ON A POLYMERIC PROTEIN PARTICLE ENCODED BY ADHE

被引：128

作者：

KESSLER, D ^{[1
]}

LEIBRECHT, I ^{[1
]}

KNAPPE, J ^{[1
]}

机构：

[1] UNIV HEIDELBERG,INST BIOL CHEM,IM NEUENHEIMER FELD 501,W-6900 HEIDELBERG,GERMANY

来源：

FEBS LETTERS | 1991年 / 281卷 / 1-2期

关键词：

FE-2+-ACTIVATION; SPIROSOME; NUCLEOTIDE SEQUENCE; ANAEROBIC INDUCTION;

D O I：

10.1016/0014-5793(91)80358-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 4.8 kb DNA-fragment was cloned and sequenced encompassing the structural gene of PFL-deactivase (2.7 kb) and 2 kb of the 5' flanking region that contains the elements for anaerobic induction. A mutant lacking deactivase was shown to require exogenous electron acceptors for anaerobic growth with glucose. This revealed the identity of PFL-deactivase with the alcohol and acetaldehyde dehydrogenases of E. coli. The multienzyme represents a homopolymeric protein (approximately 40 x 96 kDa) requiring Fe2+ for all functions.

引用

页码：59 / 63

页数：5

共 16 条

[1] THE FERMENTATION PATHWAYS OF ESCHERICHIA-COLI [J].

CLARK, DP .

FEMS MICROBIOLOGY LETTERS, 1989, 63 (03) :223-234

[2] PYRUVATE FORMATE-LYASE (INACTIVE FORM) AND PYRUVATE FORMATE-LYASE ACTIVATING ENZYME OF ESCHERICHIA-COLI - ISOLATION AND STRUCTURAL-PROPERTIES [J].

CONRADT, H ;

HOHMANNBERGER, M ;

HOHMANN, HP ;

BLASCHKOWSKI, HP ;

KNAPPE, J .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1984, 228 (01) :133-142

[3] CLONING AND SEQUENCE-ANALYSIS OF THE FERMENTATIVE ALCOHOL-DEHYDROGENASE-ENCODING GENE OF ESCHERICHIA-COLI [J].

GOODLOVE, PE ;

CUNNINGHAM, PR ;

PARKER, J ;

CLARK, DP .

GENE, 1989, 85 (01) :209-214

[4] IDENTIFICATION OF CLONES THAT ENCODE CHICKEN TROPOMYOSIN BY DIRECT IMMUNOLOGICAL SCREENING OF A CDNA EXPRESSION LIBRARY [J].

HELFMAN, DM ;

FERAMISCO, JR ;

FIDDES, JC ;

THOMAS, GP ;

HUGHES, SH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (01) :31-35

[5]

KASHIWAGI K, 1990, J BIOL CHEM, V265, P8387

[6]

KESSLER D, 1988, 14TH INT C BIOCH PRA

[7] THE PHYSICAL MAP OF THE WHOLE ESCHERICHIA-COLI CHROMOSOME - APPLICATION OF A NEW STRATEGY FOR RAPID ANALYSIS AND SORTING OF A LARGE GENOMIC LIBRARY [J].

KOHARA, Y ;

AKIYAMA, K ;

ISONO, K .

CELL, 1987, 50 (03) :495-508

[8]

MATAYOSHI S, 1989, J GEN MICROBIOL, V135, P525

[9]

NAPPE J, 1990, FEMS MICROBIOL REV, V75, P383

[10]

PASCAL MC, 1981, J GEN MICROBIOL, V124, P35

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