EFFECTIVE SEPARATION OF THE 52KDA SSA/RO POLYPEPTIDE FROM THE 48KDA SSB/LA POLYPEPTIDE BY ALTERING CONDITIONS OF POLYACRYLAMIDE-GEL ELECTROPHORESIS

被引：77

作者：

BUYON, JP ^{[1
]}

SLADE, SG ^{[1
]}

CHAN, EKL ^{[1
]}

TAN, EM ^{[1
]}

WINCHESTER, R ^{[1
]}

机构：

[1] SCRIPPS CLIN & RES FDN,WM KECK AUTOIMMUNE DIS CTR,LA JOLLA,CA 92037

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 129卷 / 02期

关键词：

Auto-antibody; SDS-PAGE; SSA/Ro; 52; kDa; SSB/La; 48;

D O I：

10.1016/0022-1759(90)90440-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide. These two proteins anomalously co-migrate with the same relative mass under conditions of conventional sodium dodecyl sulfate polyacrylamide gel electrophoresis according to Laemmli using a stock solution of acrylamide: bisacrylamide 30:08, ratio = 37.5. A higher ratio of monomer to cross-linker, ratio = 172.4 used in a 15% acrylamide solution, readily separates the two peptide chains. This method facilitates the detection of the 52 kDa SSA/Ro component which otherwise might have been incorrectly assigned as a 48 kDa SSB/La polypeptide. © 1990.

引用

页码：207 / 210

页数：4

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