HEME OXYGENASE ACTIVITY IN THE ADULT-RAT AORTA AND LIVER AS MEASURED BY CARBON-MONOXIDE FORMATION

被引：85

作者：

COOK, MN

NAKATSU, K

MARKS, GS

MCLAUGHLIN, BE

VREMAN, HJ

STEVENSON, DK

BRIEN, JF

机构：

[1] QUEENS UNIV,FAC MED,DEPT PHARMACOL & TOXICOL,KINGSTON,ON K7L 3N6,CANADA

[2] STANFORD UNIV,SCH MED,DEPT PEDIAT,STANFORD,CA 94305

来源：

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | 1995年 / 73卷 / 04期

关键词：

CARBON MONOXIDE; HEME OXYGENASE; VASCULAR SMOOTH MUSCLE; LIVER; GAS CHROMATOGRAPHIC ANALYSIS;

D O I：

10.1139/y95-065

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Rat aorta was homogenized and the 13000 x g supernatant fraction was tested for heme oxygenase (HO) activity by using a sensitive gas chromatographic method to measure carbon monoxide (GO), one of the products of the HO reaction. The rate of NADPH-dependent CO formation, an index of HO activity, was 1.41 +/- 0.40 nmol CO . mg(-1) protein . h(-1) in the rat aorta supernatant fraction and 2.05 +/- 0.55 nmol CO . mg(-1) protein . h(-1) in the rat liver 13000 X g supernatant fraction, a tissue known to contain HO activity. Chromium mesoporphyrin (0.05 mM), an inhibitor of rat liver HO, significantly decreased HO activity by 26% in the aorta supernatant fraction and 50% in the liver supernatant fraction. On the basis of the results of this study, which demonstrated HO-catalyzed CO formation in aortic tissue, and previous observations that CO relaxes vascular smooth muscle, we suggest that a physiological role for CO in vascular smooth muscle relaxation should be further investigated.

引用

页码：515 / 518

页数：4

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