IMPROVED DETECTION OF LOSS OF HETEROZYGOSITY AT RETINOBLASTOMA GENE LOCUS IN HUMAN BREAST-CARCINOMA

被引：9
作者：
TAMURA, G [1 ]
MAESAWA, C [1 ]
SUZUKI, Y [1 ]
KASHIWABA, M [1 ]
ISHIDA, M [1 ]
SAITO, K [1 ]
SATODATE, R [1 ]
机构：
[1] IWATE UNIV,SCH MED,DEPT SURG,MORIOKA,IWATE 020,JAPAN
来源：
PATHOLOGY INTERNATIONAL | 1994年 / 44卷 / 01期
关键词：
BREAST CARCINOMA; CELL SORTING; LOSS OF HETEROZYGOSITY; POLYMERASE CHAIN REACTION SINGLE-STRAND CONFORMATION POLYMORPHISM ANALYSIS; RETINOBLASTOMA GENE;
D O I：
10.1111/j.1440-1827.1994.tb02583.x
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
Loss of heterozygosity (LOH) at the retinoblastoma (Rb) gene locus was investigated in 33 breast carcinomas by polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) analysis after tumor cell enrichment by cell sorting. The efficacy of cell sorting was evaluated by comparing the results of PCR-SSCP with and without cell sorting. Ten of 17 (59%) informative cases showed LOH at this locus by cell sorting combined with PCR-SSCP, although LOH was detectable in six (35%) cases without cell sorting. Flow cytometry and histologic examination revealed that this underestimation may occur when the tumor cell population is less than 50% in the specimens analyzed. It is concluded that LOH of the Rb gene occurs more frequently in human breast carcinoma than previously thought, and thus may contribute significantly to the development and/or progression of this tumor.
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收藏
页码：34 / 38
页数：5
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