ISOLATION AND CHARACTERIZATION OF A MAIZE GENE ENCODING CHALCONE FLAVONONE ISOMERASE

被引:27
作者
GROTEWOLD, E
PETERSON, T
机构
来源
MOLECULAR & GENERAL GENETICS | 1994年 / 242卷 / 01期
关键词
ZEA MAYS; FLAVONOID BIOSYNTHESIS; P GENE;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report here the first cloning of a chalcone flavonone isomerase gene (CHI) from maize. Northern blot experiments indicate that the maize CHI gene (ZmCHI1) is regulated in the pericarp by the P gene, a myb homologue. The ZmCHI1 gene encodes a 24.3 kDa product 55% and 58% identical to CHI-A and CHI-B from Petunia, respectively. This maize CHI gene has four exons and an intron-exon structure identical to the CHI-B gene of Petunia hybrida. RFLP mapping data indicate that some inbred lines contain two additional CHI-hemologous sequences, suggesting an organization more complex than that found in Petunia or bean. The possibility that the additional CHI-homologous sequences are responsible for the lack of CHI mutants in maize will be discussed.
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页码:1 / 8
页数:8
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