DISCRIMINATION BETWEEN ALPHA-SATELLITE DNA-SEQUENCES FROM CHROMOSOME-21 AND CHROMOSOME-13 BY USING POLYMERASE CHAIN-REACTION

被引：11

作者：

CHARLIEU, JP ^{[1
]}

MURGUE, B ^{[1
]}

LAURENT, AM ^{[1
]}

MARCAIS, B ^{[1
]}

BELLIS, M ^{[1
]}

ROIZES, G ^{[1
]}

机构：

[1] INST BIOL,INSERM,U249,F-34060 MONTPELLIER,FRANCE

来源：

GENOMICS | 1992年 / 14卷 / 02期

关键词：

D O I：

10.1016/S0888-7543(05)80256-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

α-Satellite subfamilies from chromosomes 21 and 13 are almost identical in sequence and cannot be distinguished from each other by hybridization techniques. A general method based on membrane-bound PCR is described here, allowing the discrimination of α-satellite DNA sequences from each of these two chromosomes, after detection by Southern blot hybridization. The PCR conditions were developed using somatic hybrid DNAs. The method was tested in membrane-bound PCR by using the α-satellite bands from a Southern blot of a CEPH family. The chromosomal origin of these bands, previously determined by linkage analysis, was confirmed by this method. © 1992 Academic Press, Inc. All rights reserved.

引用

页码：515 / 516

页数：2

共 10 条

[1] ALPHOID DNA POLYMORPHISMS FOR CHROMOSOME-21 CAN BE DISTINGUISHED FROM THOSE OF CHROMOSOME-13 USING PROBES HOMOLOGOUS TO BOTH [J].