Formulation development and characterization of polymeric nanoparticles of antituberculosis drug Isoniazid

The objective of this study was to develop nanoparticles of Isoniazid, enhancement of bioavailability and reduction in side effects as an antituberculosis drug. Nanoparticles were prepared by Ionotropic gelation method using chitosan as polymer and tripolyphosphate crosslinking agent. Nanoparticles were characterized for particle size, surface morphology, entrapment efficiency, zeta potential and in-vitro drug release. The optimized formulation exhibited a particle size of 412.9 +/- 0.28 nm; drug entrapment efficiency of 67.40 +/- 0.59% and the in-vitro release was 91.87% extended up to 8 hrs. Stability study at 5 +/- 1 degrees C and room temperature (25 degrees C) showed that there was no significant alteration in physical appearance, size, shape, drug content and in-vitro drug release after 45 days.