HISTOCHEMICAL STUDIES ON GLYCOGEN SYNTHETASE ACTIVITY BY AUTORADIOGRAPHIC METHOD

Glycogen synthetase (uridine diphosphate glucose-glycogen glucosyl transferase) was studied in different organs by a histoautoradiographic method and by usual staining methods. This activity was found to be present in muscles and liver of different animals. Human skin also showed some activity. Human liver and myocardium showed the highest activity. In the present study, it was found that the glucose-6-phosphate dependent form (D-form) of the glycogen synthetase predominates over the glucose-6-phosphate independent form (I-form) in all the organs except hamster liver where the I-form predominates. Addition of calcium chloride in the incubation medium, to prevent phosphorolytic breakdown of the newly synthesized glycogen, does not improve the reaction. No glucose is incorporated into glycogen from 14C-glucose-6-phosphate of the incubation medium for glycogen synthetase. Fixation in absolute alcohol at -20° is recommended for tissues where cytolysis is caused by the incubation medium. © 1968 Springer-Verlag.