PROTHYMOSIN ALPHA-1 MODULATES LYMPHOKINE-ACTIVATED KILLER-CELL ACTIVITY AND IL-2 PRODUCTION BY PERIPHERAL-BLOOD LYMPHOCYTES FROM MELANOMA PATIENTS IN-VITRO

The effects of prothymosin alpha l (Pro alpha l) on the natural killer (NK), lymphokine (IL-2)-activated killer (LAK) cell activity and the phytohaemagglutinin (PHA)-induced IL-2 secretion of peripheral blood T-lymphocytes (PBL) from 34 malignant melanoma patients of all clinical stages were studied in vitro. On average, melanoma patients showed lower NK and LAK cell activities than healthy donors. In particular, patients with metastases revealed an impaired NK cell activity. However, individuals showed a broad range of LAK cell sensitivity to Pro al depending, among other factors, on the disease stage. LAK cell activities were not correlated to tumour stage. Patients' impaired LAK cell activity could be restored by Pro alpha l. Only patients at stage II (regional metastases) responded to Pro alpha l. The IL-2 secretion from PBL melanoma and healthy donors did not differ; Pro alpha l administration was without any significant effect. However, stage III (distant metastases) PBL expressed significantly lower IL-2 levels, compared to stage I (primary rumours). The highest IL-2 level was found to be associated with tumour stage II. Pro alpha l enhanced the IL-2 secretion from stage I PBL. Therefore Pro alpha l administration abrogated the defective LAK cell activity and IL-2 secretion of PBL, mainly from patients at early melanoma stages.