IDENTIFICATION OF MAJOR PROTEINS ASSOCIATED WITH DICTYOSTELIUM-DISCOIDEUM ENDOCYTIC VESICLES

被引：0

作者：

ADESSI, C

CHAPEL, A

VINCON, M

RABILLOUD, T

KLEIN, G

SATRE, M

GARIN, J

机构：

[1] CEA,INSERM,BIOL MOLEC CYCLE CELLULAIRE LAB,F-38054 GRENOBLE,FRANCE

[2] CEA,CTR ETUD NUCL,BIOL CELLULAIRE LAB,CNRS,URA 1130,F-38054 GRENOBLE,FRANCE

来源：

JOURNAL OF CELL SCIENCE | 1995年 / 108卷

关键词：

V-ATPASE; RAB; CYSTEINE PROTEINASE; DICTYOSTELIUM DISCOIDEUM; ENDOCYTOSIS; MICROSEQUENCE ANALYSIS;

D O I：

暂无

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Magnetic isolation of endocytic vesicles from Dictyostelium discoideum was accomplished after feeding the amoebae with iron oxide particles. Proteins associated with the endocytic vesicles were resolved by SDS-PAGE and digested 'in-gel' with endoproteinase Lys-C or Asp-N to generate peptides for amino acid sequencing, This strategy allowed the identification of the major protein constituents of the vesicles: namely, the A, B, D, E and 110 kDa subunits of a vacuolar type H+-ATPase, actin, a Rab 7-like GTPase, a p34 protein corresponding to a new cysteine proteinase and the 25 kDa product of a recently sequenced D. discoideum open reading frame.

引用

页码：3331 / 3337

页数：7

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