AN INSERTIONAL POINT MUTATION INACTIVATES NOLR REPRESSOR IN RHIZOBIUM-MELILOTI 1021

被引:25
作者
CREN, M
KONDOROSI, A
KONDOROSI, E
机构
[1] CNRS,INST SCI VEGETALES,BATIMENT 23,AVE TERRASSE,F-91198 GIF SUR YVETTE,FRANCE
[2] HUNGARIAN ACAD SCI,BIOL RES CTR,INST GENET,H-6701 SZEGED,HUNGARY
关键词
D O I
10.1128/JB.176.2.518-519.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In the majority of Rhizobium meliloti isolates, nod gene expression is controlled by NolR but this is not the case in a few strains including the widely used laboratory strain 1021. In 1021, the lack of NolR function was shown to be due to a single insertional mutation in the C-terminal coding sequence which abolished the DNA-binding ability, though the helix-turn-helix motif remained intact. This indicates that the C-terminal part of the protein is also essential for DNA binding. We conclude that in this species, control of nod gene expression involves NolR and strain 1021 represents an exception in which the NolR function was lost by a single event.
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页码:518 / 519
页数:2
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