REGULATION OF PHYTOCHROME-A MESSENGER-RNA ABUNDANCE IN PARSLEY SEEDLINGS AND CELL-SUSPENSION CULTURES

被引：7

作者：

POPPE, C ^{[1
]}

EHMANN, B ^{[1
]}

FROHNMEYER, H ^{[1
]}

FURUYA, M ^{[1
]}

SCHAFER, E ^{[1
]}

机构：

[1] UNIV FREIBURG,INST BIOL 2,D-79104 FREIBURG,GERMANY

来源：

PLANT MOLECULAR BIOLOGY | 1994年 / 26卷 / 01期

关键词：

PHYTOCHROME; PARSLEY; LIGHT REGULATION; TRANSIENT GENE EXPRESSION;

D O I：

10.1007/BF00039558

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone encoding the apoprotein of a parsley phytochrome was isolated and classified as parsley PHYA phytochrome, on the basis of a sequence homology comparison with all available phytochrome sequences. Red light pulses led to a phytochrome-dependent down-regulation of PHYA mRNA abundance in etiolated parsley seedlings to a level of 10-20% compared with the dark control. The PHYA mRNA abundance in a parsley cell suspension culture was also down-regulated by light pulses. Transient expression assays in parsley protoplasts showed light regulation of a chimeric pea PHYA promoter uidA-gene construct.

引用

页码：481 / 486

页数：6