GENETIC MANIPULATION OF CAMPYLOBACTER - EVALUATION OF NATURAL TRANSFORMATION AND ELECTROTRANSFORMATION

被引：91

作者：

WASSENAAR, TM ^{[1
]}

FRY, BN ^{[1
]}

VANDERZEIJST, BAM ^{[1
]}

机构：

[1] UNIV UTRECHT,SCH VET MED,INST INFECT DIS & IMMUNOL,DEPT BACTERIOL,POB 80165,3508 TD UTRECHT,NETHERLANDS

来源：

GENE | 1993年 / 132卷 / 01期

关键词：

CAMPYLOBACTER-JEJUNI; SUICIDE VECTOR; HOMOLOGOUS RECOMBINATION; GENE INACTIVATION; ELECTROPORATION; NATURAL COMPETENCE;

D O I：

10.1016/0378-1119(93)90525-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two methods, natural transformation and electro-transformation, for the introduction of DNA into nine strains of Campylobacter jejuni were compared. Both methods were successful with a limited number of strains. Natural transformation as efficient only for the introduction of C. jejuni chromosomal DNA, while electro-transformation was also applicable for the introduction of Escherichia coli-derived vector DNA into at least one C. jejuni strain. The efficiency of DNA recombination after entry was determined using C. jejuni chromosomal DNA containing disrupted flagellin genes of C. jejuni or suicide vectors containing a portion of these genes. In the latter case, DNA recombination occurred with as little as 200-bp homology present, indicating that only short homologous DNA segments are required.

引用

页码：131 / 135

页数：5

共 22 条

[1] ANALYSIS OF THE ROLE OF FLAGELLA IN THE HEAT-LABILE LIOR SEROTYPING SCHEME OF THERMOPHILIC CAMPYLOBACTERS BY MUTANT ALLELE EXCHANGE [J].