COMPARISON OF THE NEUTRAL RED AND METHYLENE-BLUE ASSAYS TO STUDY CELL-GROWTH IN CULTURE

被引：60
作者：
ELLIOTT, WM
AUERSPERG, N
机构：
[1] Department of Anatomy, University of British Columbia, Vancouver, BC V6T 123
来源：
BIOTECHNIC & HISTOCHEMISTRY | 1993年 / 68卷 / 01期
基金：
英国医学研究理事会;
关键词：
METHYLENE BLUE; NEUTRAL RED; CELL GROWTH;
D O I：
10.3109/10520299309105573
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The neutral red and methylene blue in vitro cytotoxicity assays were compared under a variety of conditions using normal human ovarian epithelial cells to determine whether either assay is superior for studying cell growth. The results were standardized against a DNA spectrofluorometric assay. Although the assays were equivalent in reflecting cell number, each has specific advantages: while neutral red discriminates between viable and dead cells, the methylene blue assay is more sensitive and easier to perform.
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页码：29 / 35
页数：7
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