AN HPLC POSTCOLUMN REACTION SYSTEM FOR PHOSPHORUS-SPECIFIC DETECTION IN THE COMPLETE SEPARATION OF INOSITOL PHOSPHATE CONGENERS IN AQUEOUS SAMPLES

被引：19

作者：

CLARKIN, CM ^{[1
]}

MINEAR, RA ^{[1
]}

KIM, S ^{[1
]}

ELWOOD, JW ^{[1
]}

机构：

[1] UNIV ILLINOIS,INST ENVIRONM STUDIES,1101 W PEABODY DR,URBANA,IL 61801

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY | 1992年 / 26卷 / 01期

关键词：

D O I：

10.1021/es00025a025

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A method for the separation of all the inositol phosphate congeners has been developed and demonstrated. Phosphorus-specific detection is achieved by postcolumn illumination by high-intensity ultraviolet radiation with resultant formation of reduced phosphomolybdate by flow injection of an ascorbic acid/molybdate reagent and spectrophotometric detection. Separations are achieved with a complex gradient between 0.1 and 0.5 N NaCl and constant concentration of Na4EDTA (0.5 mM). Submilligram per liter levels of phosphorus can be detected with 50-250-mu-L injections. Preliminary results with field samples are presented along with a proposed sample concentration procedure necessary to obtain sufficient phosphorus levels.

引用

页码：199 / 204

页数：6

共 33 条

[1] A REVIEW OF REACTION DETECTION IN HPLC
BRINKMAN, UAT
[J]. CHROMATOGRAPHIA, 1987, 24 : 190 - 200
[2] ANALYSIS OF PHYTIC ACID IN FOODS BY HPLC
CAMIRE, AL
CLYDESDALE, FM
[J]. JOURNAL OF FOOD SCIENCE, 1982, 47 (02) : 575 - 578
[3] PHYTIC ACID INTERACTIONS IN FOOD SYSTEMS
CHERYAN, M
[J]. CRC CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 1980, 13 (04): : 297 - 335
[4] CILLIERS JJL, 1986, J AGR FOOD CHEM, V35, P680
[5] COSGROVE D. J., 1967, P216
[6] Dalal R. C., 1977, Advances in Agronomy, V29, P83
[7] CHROMATOGRAPHIC INVESTIGATION OF INOSITOL PHOSPHATE ESTERS IN LAKE WATERS
EISENREICH, SJ
ARMSTRONG, DE
[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1977, 11 (05) : 497 - 501
[8] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF PHYTATE
GRAF, E
DINTZIS, FR
[J]. ANALYTICAL BIOCHEMISTRY, 1982, 119 (02) : 413 - 417
[9] ENZYMATIC CHARACTERIZATION OF SOLUBLE ORGANIC PHOSPHORUS IN LAKE WATER
HERBES, SE
ALLEN, HE
MANCY, KH
[J]. SCIENCE, 1975, 187 (4175) : 432 - 434
[10] HIXSON SW, 1977, THESIS U TENNESSEE K

← 1 2 3 4 →