DETERMINATION OF ERYTHROCYTIC POLYAMINES BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY

被引：0
|
作者：
GERBAUT, L
机构：
来源：
CLINICAL CHEMISTRY | 1991年 / 37卷 / 12期
关键词：
SPERMIDINE; SPERMINE;
D O I：
暂无
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
A simple and rapid semiautomated procedure for determining polyamines in erythrocytes by high-performance liquid chromatography is described. Putrescine, spermidine, and spermine are converted to fluorescent dansyl derivatives, extracted with cyclohexane, and separated in < 10 min on a reversed-phase C18 ODS column, with an acetonitrile-water gradient as the mobile phase. The method showed a coefficient of variation of 2.73% for spermidine and 3.27% for spermine. The respective reference values, evaluated in 10 healthy patients, were 7.88 (SD 2.09) and 5.42 (SD 1.55)-mu-mol/L of packed erythrocytes. Only negligible amounts of putrescine were found.
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页码：2117 / 2120
页数：4
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