COMPLETE NUCLEOTIDE-SEQUENCE OF TN1721 - GENE ORGANIZATION AND A NOVEL GENE-PRODUCT WITH FEATURES OF A CHEMOTAXIS PROTEIN

被引：116

作者：

ALLMEIER, H ^{[1
]}

CRESNAR, B ^{[1
]}

GRECK, M ^{[1
]}

SCHMITT, R ^{[1
]}

机构：

[1] UNIV REGENSBURG, LEHRSTUHL GENET, W-8400 REGENSBURG, GERMANY

来源：

GENE | 1992年 / 111卷 / 01期

关键词：

TRANSPOSON-BORNE GENES; S1-MAPPING; SIGMA-28-LIKE PROMOTER; CHEMOTAXIS; SIGNAL TRANSDUCTION; TRANSPOSON EVOLUTION;

D O I：

10.1016/0378-1119(92)90597-I

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The complete 11 139-nucleotide sequence of transposon Tn1721 has been determined. It contains three 38-bp inverted repeats, and (in this order) a new orfI, a resolution site (res), genes encoding resolvase (tnpR), transposase (tnpA), tetracycline-resistance (Tc(R)) repressor (tetR), Tc(R) (tetA) and a truncated transposase gene (tnpA'). The modular origin of Tn1721 from at least three separate sources is supported by the distinctive codon usages of orfI, tnpR/tnpA and tetR/tetA, and by sequence similarities with Tn501 (tnpR/tnpA) and RP1 (tetR/tetA). The ORFI-encoded 56-kDa polypeptide exhibits features of a methyl-accepting chemotaxis protein (MCP) with a conserved signal domain and a potential transmembrane domain; this polypeptide cross-reacts with anti-MCP antiserum. Like chemotaxis genes, orfI is transcribed from a sigma-28-like promoter. The overexpressed orfI gene product interferes with MCP-dependent chemotaxis suggesting that it completes for soluble transducer protein(s) in the cell. The potential selective advantage of this novel transposon-borne gene is discussed.

引用

页码：11 / 20

页数：10

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