INDIRECT FLUORESCENCE DETECTION IN MICELLAR ELECTROKINETIC CHROMATOGRAPHY

被引：44

作者：

AMANKWA, LN ^{[1
]}

KUHR, WG ^{[1
]}

机构：

[1] UNIV CALIF RIVERSIDE,DEPT CHEM,RIVERSIDE,CA 92521

来源：

ANALYTICAL CHEMISTRY | 1991年 / 63卷 / 17期

关键词：

D O I：

10.1021/ac00017a015

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Detection of neutral analytes separated by micellar electrokinetic capillary chromatography (MECC) has been accomplished by indirect fluorescence detection using quinine sulfate as the visualizing agent. The separation buffer was composed of 100.0 mM sodium dodecyl sulfate (SDS) and 0.50 mM quinine sulfate at a pH of 6.8. The detection mechanism involves a combination of displacement of the fluorophore from the micelle by the analytes and net reduction of the quantum efficiency of the fluorophore in the sample zone. The selectivity of this detection technique is demonstrated with data showing the separation of several aliphatic alcohols and some phenolic compounds. The effect of quenching by the analyte on the sensitivity of detection is also discussed.

引用

页码：1733 / 1737

页数：5

共 29 条

[1] FLUORESCENCE DETECTION IN CAPILLARY ELECTROPHORESIS - EVALUATION OF DERIVATIZING REAGENTS AND TECHNIQUES [J].