FELINE SYNCYTIUM-FORMING VIRUS - IDENTIFICATION OF A VIRION ASSOCIATED REVERSE-TRANSCRIPTASE AND ELECTRON MICROSCOPICAL OBSERVATIONS OF INFECTED-CELLS

被引:4
作者
CHISWELL, DJ
PRINGLE, CR
机构
关键词
D O I
10.1099/0022-1317-43-2-429
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The maturation of feline syncytium-forming virus (FSFV), a member of the foamy virus sub-family (Spumavirinae), has been studied by electron microscopy of thin sections of infected feline embryo (FEA) cells. The initial event observed was formation of crescent-shaped nucleoids at the plasma membrane. As budding progressed, the nucleoid became circular in outline with an electron-lucent centre in fully mature extracellular particles. These observations suggested that the maturation of FSFV in fully permissive FEA cells resembled that of C-type RNA tumour viruses, rather than the B-type mouse mammary tumour virus. In this respect FSFV may be distinct from other foamy viruses. However, like other foamy viruses FSFV possessed reverse transcriptase activity. Polymerase activity co-sedimented with infectivity in an equilibrium density gradient and exhibited a preference for poly(rA).oligo(dT)10 over poly(dA).oligo(dT)10 as exogenous template.
引用
收藏
页码:429 / 434
页数:6
相关论文
共 26 条