INTERACTION OF SHORT OLIGONUCLEOTIDE DERIVATIVES WITH NUCLEIC-ACIDS .1. EFFECT OF VARIOUS TYPE EFFECTORS

It was shown that the tandem of the derivatives of short oligonucleotides efficiently and site specifically interacts with target 20 base deoxyribonucleotide (M). It was demonstrated that the very low hybridization ability of tetranucleotide (D) and its 3'-cholesterol and 3'-estrone esters (D-ChS and D-EsS, respectively) increases significantly in the presence of the effecters: octanucleotides (E(1) and E(2)), and their 5',3'-diphenazinium (Phn-E(1)-Phn and Phn-E(2)-Phn) and 5'-cholesteryl-3'-phenazinium (ChS-E(1)-Phn and ChS-E(2)-Phn) derivatives, which flank them on the target strand. The influence of the effecters on the interaction of the target M with tetranucleotide D or its alkylating derivatives (RCl-D) increases in a series E(1) + E(2) < ChS-E(1)-Phn + ChS-E(2)-Phn < Phn-E(1)-Phn + Phn-E(2)-Phn. For the steroid derivatives, D-ChS and D-EsS, and the reagents based on them (RCl-D-ChS and RCl-D-EsS), this series is E(1) + E(2) < Phn-E(1)-Phn + Phn-E(2)-Phn < ChS-E(1)-Phn + ChS-E(2)-Phn. The modification level of the target M with derivatives RCl-D-EsS in the presence of ChS-E(1)-Phn and ChS-E(2)-Phn reaches 40% even at 37 degrees C under conditions close to physiological. The possibility of using 5'-cholesteryl-3'-phenazinium-containing oligonucleotides as effecters of the interaction of target DNA with the derivatives of short oligonucleotides was demonstrated.