An Efficient and Reliable Method for Determining the Number of Transgenes Inserted into Transgenic Plants

被引：5
作者：
Sanders, Rick A. [1 ]
Sheehy, Raymond E. [1 ]
Martineau, Belinda [1 ]
机构：
[1] Calgene Inc, Davis, CA 95616 USA
来源：
PLANT MOLECULAR BIOLOGY REPORTER | 1992年 / 10卷 / 02期
关键词：
Transgenic plants; transgene copy number analysis; polygalacturonase;
D O I：
10.1007/BF02668343
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple and reliable method is described for determining the number of transgenes inserted into transgenic plants. Analysis of tomato plants transformed with an antisense polygalacturonase gene is used to demonstrate the method. The use of the method to analyze other plants transformed with endogenous plant gene coding regions or promoters is discussed.
引用
收藏
页码：164 / 172
页数：9
相关论文
共 10 条
  • [1] NUCLEOTIDE-SEQUENCE OF THE T-DNA REGION FROM THE AGROBACTERIUM-TUMEFACIENS OCTOPINE TI PLASMID PTI15955
    BARKER, RF
    IDLER, KB
    THOMPSON, DV
    KEMP, JD
    [J]. PLANT MOLECULAR BIOLOGY, 1983, 2 (06) : 335 - 350
  • [2] NUCLEOTIDE-SEQUENCE AND EXACT LOCALIZATION OF THE NEOMYCIN PHOSPHOTRANSFERASE GENE FROM TRANSPOSON TN5
    BECK, E
    LUDWIG, G
    AUERSWALD, EA
    REISS, B
    SCHALLER, H
    [J]. GENE, 1982, 19 (03) : 327 - 336
  • [3] GENETICS OF ACTIN-RELATED SEQUENCES IN TOMATO
    BERNATZKY, R
    TANKSLEY, SD
    [J]. THEORETICAL AND APPLIED GENETICS, 1986, 72 (03) : 314 - 321
  • [4] THE TOMATO POLYGALACTURONASE GENE AND RIPENING-SPECIFIC EXPRESSION IN TRANSGENIC PLANTS
    BIRD, CR
    SMITH, CJS
    RAY, JA
    MOUREAU, P
    BEVAN, MW
    BIRD, AS
    HUGHES, S
    MORRIS, PC
    GRIERSON, D
    SCHUCH, W
    [J]. PLANT MOLECULAR BIOLOGY, 1988, 11 (05) : 651 - 662
  • [5] EFFICIENT TRANSFER OF A GLYPHOSATE TOLERANCE GENE INTO TOMATO USING A BINARY AGROBACTERIUM-TUMEFACIENS VECTOR
    FILLATTI, JJ
    KISER, J
    ROSE, R
    COMAI, L
    [J]. BIO-TECHNOLOGY, 1987, 5 (07): : 726 - 730
  • [6] T-DNA IS ORGANIZED PREDOMINANTLY IN INVERTED REPEAT STRUCTURES IN PLANTS TRANSFORMED WITH AGROBACTERIUM-TUMEFACIENS C58 DERIVATIVES
    JORGENSEN, R
    SNYDER, C
    JONES, JDG
    [J]. MOLECULAR AND GENERAL GENETICS, 1987, 207 (2-3): : 471 - 477
  • [7] DUPLICATION OF CAMV-35S PROMOTER SEQUENCES CREATES A STRONG ENHANCER FOR PLANT GENES
    KAY, R
    CHAN, A
    DALY, M
    MCPHERSON, J
    [J]. SCIENCE, 1987, 236 (4806) : 1299 - 1302
  • [8] Maniatis T., 1982, MOL CLONING LAB MANU
  • [9] REDUCTION OF POLYGALACTURONASE ACTIVITY IN TOMATO FRUIT BY ANTISENSE RNA
    SHEEHY, RE
    KRAMER, M
    HIATT, WR
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) : 8805 - 8809
  • [10] MOLECULAR CHARACTERIZATION OF TOMATO FRUIT POLYGALACTURONASE
    SHEEHY, RE
    PEARSON, J
    BRADY, CJ
    HIATT, WR
    [J]. MOLECULAR & GENERAL GENETICS, 1987, 208 (1-2): : 30 - 36
1