FAST PURIFICATION OF PHASEOLUS-VULGARIS ISOLECTINS

被引：5
|
作者：
ZENTENO, E
ORTEGA, M
QIN, Z
MONTREUIL, J
DEBRAY, H
机构：
[1] UNIV AUTONOMA ESTADO MORELOS,BIOL EXPTL LAB,MEXICO CITY,DF 62170,MEXICO
[2] PENN STATE UNIV,MILTON S HERSHEY MED CTR,DEPT BIOL CHEM,HERSHEY,PA 17033
[3] NATL AUTONOMOUS UNIV MEXICO,FAC MED,DEPT BIOQUIM,MEXICO CITY 04510,DF,MEXICO
来源：
PREPARATIVE BIOCHEMISTRY | 1994年 / 24卷 / 3-4期
关键词：
D O I：
10.1080/10826069408010091
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Phytohemagglutinin from red kidney bean has been purified by affinity chromatography on a human alpha(1)-acid glycoprotein Sepharose 4B column. Further purification of the hemagglutinin's five isolectins was achieved on a Mono S column with an 86% protein recovery. Each sequentially eluted isolectin from the ion exchange column displayed either hemagglutinating or mitogenic activity. The main activity of each fraction was the result of the combination of varying proportions of the L and E subunits.
引用
收藏
页码：175 / 183
页数：9
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