REGENERATION OF ELAEAGNUS-ANGUSTIFOLIA FROM LEAF SEGMENTS OF IN VITRO-DERIVED SHOOTS

Shoot regeneration was achieved from in vitro-produced leaves of Elaeagnus angustifolia L. Half-leaf explants from the terminal part of the shoot produced more shoots than explants from the basal part of the in vitro-derived shoots on agar-solidified WPM medium supplemented with 1 mu M benzyladenine (BA), In liquid medium of the same formulation, compact shoots that did not elongate were formed on the explants. Leaf cross-section explants (1 mm thick) produced shoots both on solid and liquid medium with 1 mu M BA, whereas again compact shoots were formed with 10 mu M BA. Further shoot development on these explants was promoted by their transfer to fresh solid medium containing 1 mu M BA and 1 mu M gibberellic acid (GA(3)).