ANALYSIS OF ALL STRATUM-CORNEUM LIPIDS BY AUTOMATED MULTIPLE DEVELOPMENT HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

被引:46
作者
BONTE, F
PINGUET, P
CHEVALIER, JM
MEYBECK, A
机构
[1] LVMH Recherche, 92752 Nanterre
来源
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 664卷 / 02期
关键词
D O I
10.1016/0378-4347(94)00480-S
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An optimized gradient enabling the separation of all stratum corneum lipids by automated multiple development on HPTLC plates is presented. An initial isocratic step separates sebum lipids. This is followed by a 25-step development using a gradient with a polarity range of methanol-water to hexane. Application to in-vivo extracted and isolated stratum corneum lipids demonstrates the possible quantification of the lipid classes with a ''one-experiment'' separation.
引用
收藏
页码:311 / 316
页数:6
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