PRESSURE AGAINST THE TUMOR CAN REDUCE THE EFFICIENCY OF PHOTOCHEMOTHERAPY

C3D2/F(1)mice with mammary carcinoma tumors growing subcutaneously on their right foot were given 10 mg/kg aluminium phthalocyanine tetrasulfonate (AlPcS(4)) by intraperitoneal injection. Twenty-four hours later these tumors were exposed to light at 680 nm. The size of the tumors was measured daily. An exposure of 135 J/cm(2) (150 mW/cm(2)) reduced the tumor growth rate so that the time needed for the tumors to reach a volume five times larger than that at the time of exposure increased from 4 days for control tumors to 15 days. However, when a glass plate was gently pressed against the tumor surface during irradiation, the effect of an identical exposure was significantly smaller. In agreement with this, microscopic studies showed that tumors exposed to laser light without any pressure applied during irradiation were more damaged than tumors receiving a slight pressure. Thus, pressure against the tumor can obviously reduce the oxygen concentration in this tumor enough to reduce the efficiency of the treatment.