ENHANCED SPORE PRODUCTION OF BACILLUS-THURINGIENSIS BY FED-BATCH CULTURE

被引：42

作者：

KANG, BC

LEE, SY

CHANG, HN

机构：

[1] KOREA ADV INST SCI & TECHNOL,BIOPROC ENGN RES CTR,TAEDUK SCI TOWN,TAEJON 305701,SOUTH KOREA

[2] KOREA ADV INST SCI & TECHNOL,DEPT CHEM ENGN,TAEJON 305701,SOUTH KOREA

来源：

BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 08期

关键词：

D O I：

10.1007/BF01021650

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fed-batch culture was carried out to increase cell mass followed by batch culture for spore production of Bacillus thuringiensis. High cell mass obtained by increasing the feeding glucose concentration in constant fed-batch culture did not proceed to spore formation. However, intermittent fed-batch culture which supported fast cell growth resulted in good sporulation during subsequent batch culture, and the maximum cell mass of 72.6 g/L and spore concentration of 1.25 x 10(10) spores/mL could be obtained.

引用

页码：721 / 726

页数：6

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