ION CHANNELS IN THE THYLAKOID MEMBRANE (A PATCH-CLAMP STUDY)

被引：50

作者：

ENZ, C ^{[1
]}

STEINKAMP, T ^{[1
]}

WAGNER, R ^{[1
]}

机构：

[1] UNIV OSNABRUCK,FACHBEREICH BIOL CHEM,POSTFACH 4469,W-4500 OSNABRUCK,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1143卷 / 01期

关键词：

CHLOROPLAST; THYLAKOID MEMBRANE; ION CHANNEL; MEMBRANE POTENTIAL; ELECTRON TRANSPORT;

D O I：

10.1016/0005-2728(93)90217-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thylakoid membranes were fused with azolectin liposomes and enlarged to giant thylakoid/liposome vesicles with diameters between 20 mum and 50 mum by a modified dehydration/rehydration procedure. Ion transport across the membrane of these giant thylakoid/liposome vesicles was investigated by the patch-clamp technique. Single-channel currents were observed and four different specific ion conductances could be distinguished. Our results indicate that these conductances can be attributed to three types of ion channel: a putative potassium channel with a conductance of 110 pS (in 100 mM KCl) a chloride channel with a conductance of 220 pS (in 100 mM KCl) and a moderate selectivity of about 4 : 1 for anions over cations. The third type of putative ion channel we observed was permeable to Mg2+ and Ca2+ ions with conductances of 20 pS for Ca2+ and 35 pS for Mg2+, respectively.

引用

页码：67 / 76

页数：10

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