REVERSED-PHASE LIQUID-CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF THE ANTIMALARIAL-DRUGS SULFADOXINE, PYRIMETHAMINE, MEFLOQUINE AND ITS MAJOR CARBOXYLIC METABOLITE IN PLASMA

被引：19

作者：

BERGQVIST, Y

ECKERBOM, S

LARSSON, H

MALEKZADEH, M

机构：

[1] Department of Clinical Chemistry, Falun Central Hospital

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 571卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80443-G

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic (HPLC) method for the simultaneous determination of sulfadoxine, pyrimethamine, mefloquine and the carboxylic metabolite of mefloquine in plasma is described. After the proteins have been precipitated with a combination of zinc sulphate and acetonitrile containing two internal standards, pyrimethamine and mefloquine are extracted as bases and sulfadoxine and the carboxylic metabolite of mefloquine as ion-pairs with tetrabutylammonium. The drugs are separated by HPLC on a 3-mu-m octadecylsilica column with ultraviolet detection at 229 nm. The method is simple and reliable and enables the simultaneous determination of the drugs in 600-mu-l plasma samples with a sensitivity suitable for standard drug monitoring purposes.

引用

页码：169 / 177

页数：9

共 9 条

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