EXTRACTION AND IDENTIFICATION OF A SERINE PROTEASE INHIBITOR FROM FLAX SEED (Linum usitatissimum L.)

被引：0
|
作者：
Eremia, Mihaela-Carmen [1 ]
Lupescu, Irina [1 ]
Savoiu, Gabriela Valeria [1 ]
Spiridon, Maria [1 ]
机构：
[1] ICCF, Natl Inst Chem Pharmaceut Res & Dev, Calea Vitan 112, Bucharest 031299, Romania
来源：
AGROLIFE SCIENTIFIC JOURNAL | 2016年 / 5卷 / 02期
关键词：
serine protease inhibitors; plant protease inhibitors; Linaceae family; trypsin inhibitor;
D O I：
暂无
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The trypsin inhibitor from common flax seeds (Linum usitatissimum trypsin inhibitor, abbreviate LUTI) is a member of the potato inhibitor I family. This was isolated and purified by ethanol fractionation, ion exchange chromatography and affinity chromatography. To determine the molecular mass of protease inhibitor we utilized gel electrophoresis in denatured conditions (SDS-PAGE). LUTI is the first serine protease-type inhibitor isolated from a plant of Linaceae family, and has a molecular mass 7000D.
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收藏
页码：65 / 70
页数：6
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