Screening and characterization of novel microsatellite loci in Lateolabrax japonicus

被引：3

作者：

Li, B. B. ^{[1
,2
]}

Li, Z. B. ^{[1
,2
]}

Shangguan, J. B. ^{[1
,2
]}

Ning, Y. F. ^{[1
,2
]}

Yuan, Y. ^{[1
,2
]}

Huang, Y. S. ^{[1
,2
]}

Mao, X. Q. ^{[1
,2
]}

机构：

[1] Jimei Univ, Coll Fisheries, Xiamen, Peoples R China

[2] Fujian Prov Key Lab Marine Fishery Resources & Ec, Xiamen, Peoples R China

来源：

GENETICS AND MOLECULAR RESEARCH | 2016年 / 15卷 / 04期

关键词：

Genetic markers; Lateolabrax japonicus; FIASCO; Microsatellites;

D O I：

10.4238/gmr15048378

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lateolabrax japonicus, an economically important species, is widely consumed in the offshore coasts of China, Korea, and Japan. We identified 10 new L. japonicus microsatellite markers, using a modified protocol of fast isolation by AFLP of sequences containing repeats. Thirty L. japonicus individuals were collected from Xiamen, China, to evaluate the degree of polymorphism. The number of identified alleles ranged from three to five. The polymorphism information content varied from 0.267 to 0.711, whereas the observed and expected heterozygosities ranged from 0.249 to 0.706 and 0.294 to 0.751, respectively. One of the 10 loci (L10) deviated from the Hardy-Weinberg equilibrium. These new microsatellite markers will provide a useful tool for the determination of population genetic structure and genetic diversity in L. japonicus.

引用

页数：5

共 26 条

[1] BOTSTEIN D, 1980, AM J HUM GENET, V32, P314
[2] Du Tao, 2013, Oceanologia et Limnologia Sinica / Hai Yang Yu Hu Chao, V44, P337
[3] Fan Jia-Jia, 2009, Yichuan, V31, P515, DOI 10.3724/SP.J.1005.2009.00515
[4] Screening potential SSR markers of the anadromous fish Coilia nasus by de novo transcriptome analysis using Illumina sequencing
Fang, D. -A.
Zhou, Y. -F.
Duan, J. -R.
Zhang, M. -Y.
Xu, D. -P.
Liu, K.
Xu, P.
Wei, Q.
[J]. GENETICS AND MOLECULAR RESEARCH, 2015, 14 (04): : 14181 - 14188
[5] Goldstein DB, 1999, MICROSATELLITES EVOL
[6] Family relationships and effective population size in a natural cohort of Atlantic cod (Gadus morhua) larvae
Herbinger, C. M.
Doyle, R. W.
Taggart, C. T.
Lochmann, S. E.
Brooker, A. L.
Wright, J. M.
Cook, D.
[J]. CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES, 1997, 54 : 11 - 18
[7] [胡自民 HU ZiMin], 2007, [中国海洋大学学报. 自然科学版, Journal of Ocean University of China], V37, P413
[8] Jackson TR, 1995, THESIS
[9] Microsatellite DNA polymorphism of Japanese sea bass (Laterolabrax japonicus) inhabiting Chinese and Japanese coasts
Jiang, X.
Yang, G.
Liao, M.
Liu, Y.
Gao, T.
Wang, D.
Wei, Q.
Zou, G.
[J]. JOURNAL OF APPLIED ICHTHYOLOGY, 2008, 24 (02) : 180 - 186
[10] Isolation and characterization of 22 polymorphic microsatellite DNA markers of Japanese sea bass (Laterolabrax japonicus)
Jiang, Xin
Liao, Meijie
Liu, Yongjian
Gao, Tianxiang
Yang, Guanpin
[J]. MOLECULAR ECOLOGY NOTES, 2007, 7 (03): : 492 - 494

← 1 2 3 →