SITES OF INTERACTION OF THE CCA END OF PEPTIDYL-TRANSFER RNA WITH 23S RIBOSOMAL-RNA

被引：137

作者：

MOAZED, D ^{[1
]}

NOLLER, HF ^{[1
]}

机构：

[1] UNIV CALIF SANTA CRUZ, SINSHEIMER LABS, SANTA CRUZ, CA 95064 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 09期

关键词：

RIBOSOMES; PEPTIDYLTRANSFERASE; SPARSOMYCIN;

D O I：

10.1073/pnas.88.9.3725

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Oligonucleotide fragments derived from the 3' CCA terminus of acylated tRNA, such as CACCA-(AcPhe), UACCA-(AcLeu), and CAACCA-(fMet), bind specifically to ribosomes in the presence of sparsomycin and methanol [Monro, R. E., Celma, M. L. & Vazquez, D. (1969) Nature (London) 222, 356-358]. All three oligonucleotides protect a characteristic set of bases in 23S rRNA from chemical probes: G2252, G2253, A2439, A2451, U2506, and U2585. A2602 shows enhanced reactivity. These account for most of the same bases that are protected when peptidyl-tRNA analogues such as AcPhe-tRNA are bound to the ribosomal P site, and correspond precisely to those bases whose protection is abolished by removal of the 3'-CA end of tRNA. We conclude that most of the observed interactions between tRNA and 23S rRNA in the 50S ribosomal P site involve the conserved CCA terminus of tRNA. Sparsomycin may inhibit protein synthesis by stabilizing interaction between the peptidyl-CCA and the 23S P site, preventing formation of the intermediate A/P hybrid state.

引用

页码：3725 / 3728

页数：4

共 17 条

[1] IDENTIFICATION OF A SITE ON 23S RIBOSOMAL-RNA LOCATED AT THE PEPTIDYL TRANSFERASE CENTER [J].

BARTA, A ;

STEINER, G ;

BROSIUS, J ;

NOLLER, HF ;

KUECHLER, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (12) :3607-3611

[2] A COMPILATION OF LARGE SUBUNIT RNA SEQUENCES PRESENTED IN A STRUCTURAL FORMAT [J].

GUTELL, RR ;

FOX, GE .

NUCLEIC ACIDS RESEARCH, 1988, 16 :R175-R269

[3] BEHAVIOUR OF ACETYLPHENYLALANYL SOLUBLE RIBONUCLEIC ACID IN POLYPHENYLALANINE SYNTHESIS [J].

HAENNI, AL ;

CHAPEVILLE, F .

BIOCHIMICA ET BIOPHYSICA ACTA, 1966, 114 (01) :135-+

[4] ALTERED RIBOSOMAL-RNA GENES IN MITOCHONDRIA FROM MAMMALIAN-CELLS WIT CHLORAMPHENICOL RESISTANCE [J].

KEARSEY, SE ;

CRAIG, IW .

NATURE, 1981, 290 (5807) :607-608

[5] INTERMEDIATE STATES IN THE MOVEMENT OF TRANSFER-RNA IN THE RIBOSOME [J].

MOAZED, D ;

NOLLER, HF .

NATURE, 1989, 342 (6246) :142-148

[6] CHLORAMPHENICOL, ERYTHROMYCIN, CARBOMYCIN AND VERNAMYCIN-B PROTECT OVERLAPPING SITES IN THE PEPTIDYL TRANSFERASE REGION OF 23S-RIBOSOMAL RNA [J].

MOAZED, D ;

NOLLER, HF .

BIOCHIMIE, 1987, 69 (08) :879-884

[7] BINDING OF TRANSFER-RNA TO THE RIBOSOMAL A-SITES AND P-SITES PROTECTS 2 DISTINCT SETS OF NUCLEOTIDES IN 16 S RIBOSOMAL-RNA [J].

MOAZED, D ;

NOLLER, HF .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 211 (01) :135-145

[8] TRANSFER-RNA SHIELDS SPECIFIC NUCLEOTIDES IN 16S RIBOSOMAL-RNA FROM ATTACK BY CHEMICAL PROBES [J].

MOAZED, D ;

NOLLER, HF .

CELL, 1986, 47 (06) :985-994

[9] INTERACTION OF ANTIBIOTICS WITH FUNCTIONAL SITES IN 16S RIBOSOMAL-RNA [J].

MOAZED, D ;

NOLLER, HF .

NATURE, 1987, 327 (6121) :389-394

[10] RIBOSOME-CATALYZED PEPTIDYL TRANSFER - SUBSTRATE SPECIFICITY AT P-SITE [J].

MONRO, RE ;

CERNA, J ;

MARCKER, KA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1968, 61 (03) :1042-&

← 1 2 →