METAL-ION CONCENTRATION, TIME, AND PH-DEPENDENCE OF METAL-ION BINDING TO A TRANSFERRIN METALLOPROTEIN AFFINITY-CHROMATOGRAPHY (MAMC) MATRIX

被引：4
|
作者：
CANNELL, KP [1 ]
VINCENT, JB [1 ]
机构：
[1] UNIV ALABAMA, DEPT CHEM, TUSCALOOSA, AL 35487 USA
来源：
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY | 1995年 / 64卷 / 01期
关键词：
TRANSFERRIN; CONALBUMIN; ADSORPTION ISOTHERMS;
D O I：
10.1002/jctb.280640115
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The adsorption of transition metal, lanthanide, and actinide ions to ovotransferrin (conalbumin) immobilized to sepharose (via the cyanogen bromide method) has been examined. Adsorption of ions as a function of time and adsorption isotherms at pH 8 have been determined and analyzed using the Freundlich model. Distribution coefficients between the pH values 2 and 9 have been measured. The results indicate that immobilization of the protein has little effect on its interactions with metal ions compared with the protein in solution, an important prerequisite for use of this matrix in metalloprotein affinity metal chromatography (MAMC).
引用
收藏
页码：96 / 100
页数：5
相关论文
共 50 条
  • [31] Methacryloylamidohistidine in Affinity Ligands for Immobilized Metal-ion Affinity Chromatography of Ferritin
    Uygun, Deniz Aktas
    Ozturk, Nevra
    Akgol, Sinan
    Denizli, Adil
    BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 2011, 16 (01) : 173 - 179
  • [32] METAL-ION BINDING TO YEAST PHOSPHOGLYCERATE KINASE - INTERACTION BETWEEN METAL-ION BINDING AND ANION BINDING
    WROBEL, JA
    STINSON, RA
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1980, 104 (01): : 249 - 254
  • [33] RAPID ONE-STEP PURIFICATION OF GOAT IMMUNOGLOBULINS BY IMMOBILIZED METAL-ION AFFINITY-CHROMATOGRAPHY
    BODEN, V
    WINZERLING, JJ
    VIJAYALAKSHMI, M
    PORATH, J
    JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 181 (02) : 225 - 232
  • [34] METAL-ION LIQUID-CHROMATOGRAPHY
    RAJA, R
    AMERICAN LABORATORY, 1982, 14 (07) : 35 - 37
  • [35] METAL-ION CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
    DASGUPTA, PK
    SOROKA, K
    VITHANAGE, RS
    JOURNAL OF LIQUID CHROMATOGRAPHY, 1987, 10 (15): : 3287 - 3319
  • [36] METAL-ION BINDING IN TRICLINIC LYSOZYME
    KURACHI, K
    SIEKER, LC
    JENSEN, LH
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1975, 250 (19) : 7663 - 7667
  • [37] METAL-ION BINDING IN TRICLINIC LYSOZYME
    KURACHI, K
    SIEKER, LC
    JENSEN, LH
    ACTA CRYSTALLOGRAPHICA SECTION A, 1975, 31 : S26 - S26
  • [38] METAL-ION BINDING TO DAUNORUBICIN AND QUINIZARIN
    KIRALY, R
    MARTIN, RB
    INORGANICA CHIMICA ACTA-BIOINORGANIC CHEMISTRY, 1982, 67 (01): : 13 - 18
  • [39] DESIGN OF METAL-ION BINDING PEPTIDES
    FATTORUSSO, R
    MORELLI, G
    LOMBARDI, A
    NASTRI, F
    MAGLIO, O
    DAURIA, G
    PEDONE, C
    PAVONE, V
    BIOPOLYMERS, 1995, 37 (06) : 401 - 410
  • [40] Immobilized metal-ion affinity chromatography of peptides on metalloporphyrin stationary phases
    Magdalena Biesaga
    Jowita Orska
    Dariusz Fiertek
    J. Izdebski
    M. Trojanowicz
    Fresenius' Journal of Analytical Chemistry, 1999, 364 : 160 - 164
12345