REVERSIBLE INHIBITION OF CYTOCHROME-C-OXIDASE, THE TERMINAL ENZYME OF THE MITOCHONDRIAL RESPIRATORY-CHAIN, BY NITRIC-OXIDE - IMPLICATIONS FOR NEURODEGENERATIVE DISEASES

被引：1103

作者：

CLEETER, MWJ

COOPER, JM

DARLEYUSMAR, VM

MONCADA, S

SCHAPIRA, AHV

机构：

[1] ROYAL FREE HOSP,SCH MED,DEPT NEUROSCI,LONDON NW3 2PF,ENGLAND

[2] ROYAL FREE HOSP,SCH MED,DEPT PROT & MOLEC BIOL,LONDON NW3 2PF,ENGLAND

[3] INST NEUROL,LONDON WC1 3BN,ENGLAND

[4] WELLCOME RES LABS,BECKENHAM BR3 3QX,KENT,ENGLAND

来源：

FEBS LETTERS | 1994年 / 345卷 / 01期

关键词：

MITOCHONDRION; PARKINSONS DISEASE; CYTOCHROME C OXIDASE;

D O I：

10.1016/0014-5793(94)00424-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation of rat skeletal muscle mitochondria with the nitric oxide generator, S-nitrosoglutathione (GSNO) reversibly inhibited oxygen utilisation with all substrates tested. The visible absorption spectra of the inhibited mitochondria showed that cytochromes c + c(1), b and a + a(3) were reduced, indicating a block at the distal end of the respiratory chain. Analysis of the respiratory chain enzyme activities in the presence of GSNO localised the site of inhibition to cytochrome c oxidase alone. These results indicate that nitric oxide is capable of rapidly and reversibly inhibiting the mitochondrial respiratory chain and may be implicated in the cytotoxic effects of nitric oxide in the CNS and other tissues.

引用

页码：50 / 54

页数：5