A NEW METHOD FOR THE CONCENTRATION OF CRYPTOSPORIDIUM OOCYSTS FROM WATER

被引：143

作者：

VESEY, G ^{[1
]}

SLADE, JS ^{[1
]}

BYRNE, M ^{[1
]}

SHEPHERD, K ^{[1
]}

FRICKER, CR ^{[1
]}

机构：

[1] THAMES WATER UTIL LTD, SPENCER HOUSE LABS, MANOR FARM RD, READING RG2 OJN, ENGLAND

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1993年 / 75卷 / 01期

关键词：

D O I：

10.1111/j.1365-2672.1993.tb03412.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel method for the concentration of Cryptosporidium oocysts from water has been developed, based upon the precipitation of calcium carbonate. A 10 1 water sample is treated by adding solutions of calcium chloride and sodium bicarbonate and raising the pH value to 10 with sodium hydroxide. Crystals of calcium carbonate form and enmesh particles in the Cryptosporidium oocyst size range. The crystals are allowed to settle, the supernatant fluid is discarded and the calcium carbonate precipitate dissolved in sulphamic acid. The sample can be concentrated further by centrifugation. Recoveries of oocysts from seeded samples of deionized, tap and river water were in excess of 68%.

引用

页码：82 / 86

页数：5

共 7 条

[1]

BADENOCH J, 1990, CRYPTOSPORIDIUM WATE

[2]

GILMOR RA, 1990, THESIS U STRATHCLYDE

[3]

LECHEVALLIER MW, 1991, AM SOC TEST MATER, V1102, P483, DOI 10.1520/STP17618S

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ONGERTH, JE ;

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[5] ISOLATION AND IDENTIFICATION OF CRYPTOSPORIDIUM FROM WATER [J].

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WATER SCIENCE AND TECHNOLOGY, 1991, 24 (02) :165-167

[6] TAKING THE EYE STRAIN OUT OF ENVIRONMENTAL CRYPTOSPORIDIUM ANALYSIS [J].

VESEY, G ;

SLADE, JS ;

FRICKER, CR .

LETTERS IN APPLIED MICROBIOLOGY, 1991, 13 (02) :62-65

[7] ROUTINE MONITORING OF CRYPTOSPORIDIUM OOCYSTS IN WATER USING FLOW-CYTOMETRY [J].

VESEY, G ;

SLADE, JS ;

BYRNE, M ;

SHEPHERD, K ;

DENNIS, PJ ;

FRICKER, CR .

JOURNAL OF APPLIED BACTERIOLOGY, 1993, 75 (01) :87-90

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