OLIGOTROPHIC PSEUDOMONADS IN THE RHIZOSPHERE - SUPPRESSIVENESS TO PYTHIUM DAMPING-OFF OF CUCUMBER SEEDLINGS (CUCUMIS-SATIVUS L)

被引:13
作者
SUGIMOTO, EE
HOITINK, HAJ
TUOVINEN, OH
机构
[1] OHIO STATE UNIV,DEPT MICROBIOL,484 W 12TH AVE,COLUMBUS,OH 43210
[2] OHIO STATE UNIV,OHIO AGR RES & DEV CTR,PLANT BIOTECHNOL PROGRAM,WOOSTER,OH 44691
[3] OHIO STATE UNIV,OHIO AGR RES & DEV CTR,DEPT PLANT PATHOL,WOOSTER,OH 44691
关键词
Biological suppression; Cucumis sativus L; Oligotrophs; Pseudomonas spp; Pythium damping-off; Rhizosphere pseudomonads;
D O I
10.1007/BF00336231
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
Pseudomonads recovered from the cucumber rhizosphere were evaluated for their ability to suppress Pythium ultimum damping-off. Full-strength and 250-fold diluted selective media formulations with King's B medium (KB and KB/250, respectively) as the basal medium were used as the recovery media. Eight per cent of the isolates recovered (11/138) could induce suppression to Pythium damping-off and 45% of these biocontrol agents produced fluorescent pigments. No differences in the ability to induce suppression among the isolates recovered on full-strength and diluted media were detected. The growth rates of the selected isolates indicated that growth in KB broth was faster or equal to that in KB/10. Therefore, no obligate oligotrophs were recovered. Additionally, growth was observed in 15 000-fold diluted KB for 10 of 11 test isolates, which indicated that these isolates were facultative oligotrophs. In general, the faster growing isolates were more likely to induce suppression when used as seed treatments. Finally, the in vitro antifungal properties of test isolates against P. ultimum indicated that 4 of the 11 isolates inhibited P. ultimum. © 1990 Springer-Verlag.
引用
收藏
页码:231 / 234
页数:4
相关论文
共 9 条
[1]  
[Anonymous], IRON TRANSPORT MICRO
[2]   THE ROLE OF MICROBIAL ACTIVITY IN SUPPRESSION OF DAMPING-OFF CAUSED BY PYTHIUM-ULTIMUM [J].
CHEN, W ;
HOITINK, HAJ ;
SCHMITTHENNER, AF ;
TUOVINEN, OH .
PHYTOPATHOLOGY, 1988, 78 (03) :314-322
[3]   SURVIVAL OF PSEUDOMONAS-PUTIDA, A BIOLOGICAL-CONTROL AGENT, IN SOIL [J].
DUPLER, M ;
BAKER, R .
PHYTOPATHOLOGY, 1984, 74 (02) :195-200
[4]  
KING EO, 1954, J LAB CLIN MED, V44, P301
[5]   BIOLOGY OF OLIGOTROPHIC BACTERIA [J].
KUZNETSOV, SI ;
DUBININA, GA ;
LAPTEVA, NA .
ANNUAL REVIEW OF MICROBIOLOGY, 1979, 33 :377-387
[6]  
KWOK OCH, 1987, PHYTOPATHOLOGY, V77, P1206, DOI 10.1094/Phyto-77-1206
[7]  
LOPER J, 1987, PHYTOPATHOLOGY, V78, P166
[8]   THE ROLE OF MICROORGANISMS IN THE SUPPRESSION OF RHIZOCTONIA-SOLANI IN CONTAINER MEDIA AMENDED WITH COMPOSTED HARDWOOD BARK [J].
NELSON, EB ;
HOITINK, HAJ .
PHYTOPATHOLOGY, 1983, 73 (02) :274-278
[9]   ENUMERATION OF OLIGOTROPHIC RHIZOSPHERE PSEUDOMONADS WITH DILUTED AND SELECTIVE MEDIA FORMULATIONS [J].
SUGIMOTO, EE ;
HOITINK, HAJ ;
TUOVINEN, OH .
BIOLOGY AND FERTILITY OF SOILS, 1990, 9 (03) :226-230