Specific Determination of E. coli B Using Bacteriophage T4, Nanofilters, and ATP Assay

被引：1

作者：

Minikh, O. A. ^{[1
,2
]}

Brovko, L. Yu ^{[1
]}

Griffiths, M. W. ^{[1
]}

Ugarova, N. N. ^{[2
]}

机构：

[1] Univ Guelph, Canadian Res Inst Food Safety, McGilvray St, Guelph, ON N1G 2W1, Canada

[2] Moscow MV Lomonosov State Univ, Fac Chem, Dept Chem Enzymol, Moscow 119991, Russia

来源：

MOSCOW UNIVERSITY CHEMISTRY BULLETIN | 2010年 / 65卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

bacteriophage T4; determination of bacterial contamination; E. coli B; bioluminescence; ATP; nanoalumina fibers;

D O I：

10.3103/S0027131410030181

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Lytic bacteriophages and "Disruptor" 4610 filters made of nanoalumina fibers were used for specific determination of determination of bacterial contamination by ATP assay. As a model system the system of bacteriophage T4-E. coli B was used as a model object. Modifications of the method allow either the quantitative detection of E. coli B with a detection limit as low as 1.0 x 10(3) CFU/ml or detection of E. coli B in the presence of 60-fold excess of S. typhimurium. Both modifications showed much better performance than the standard methods based on the use of bacteriophage T4. The increase in testing time was insignificant.

引用

页码：202 / 205

页数：4

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