NUCLEAR MATRIX ATTACHMENT REGIONS AND TOPOISOMERASE-II BINDING AND REACTION SITES IN THE VICINITY OF A CHICKEN DNA-REPLICATION ORIGIN

被引：55
|
作者：
RAZIN, SV [1 ]
VASSETZKY, YS [1 ]
HANCOCK, R [1 ]
机构：
[1] UNIV LAVAL,HOTEL DIEU HOSP,CTR RECH CANCEROL,QUEBEC CITY G1R 2J6,QUEBEC,CANADA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 177卷 / 01期
基金：
英国医学研究理事会;
关键词：
D O I：
10.1016/0006-291X(91)91977-K
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have mapped nuclear matrix attachment regions (MARs), defined by their specific binding to nuclear matrices in vitro, and sites of topoisomerase II reaction, detected by DNA cleavage in vitro in the presence of the inhibitor VM-26, in the vicinity of the replication origin of the chicken α-globin gene domain. Two MARs are located close to the downstream end (in the direction of transcription) of a 3 kb fragment which includes the origin. These MARs contain sites for strong topoisomerase II binding and reaction. Our observations on this gene domain support two hypotheses concerning MARs in eukaryotic cells, namely that they are close to DNA replication origins and that they contain multiple topoisomerase II recognition sites. © 1991.
引用
收藏
页码：265 / 270
页数：6
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