PRESENCE OF PARALYTIC SHELLFISH POISONING TOXINS AND SOLUBLE-PROTEINS IN TOXIC BUTTER CLAMS (SAXIDOMUS-GIGANTEUS)

被引：6

作者：

KITTS, DD ^{[1
]}

SMITH, DS ^{[1
]}

BEITLER, MK ^{[1
]}

LISTON, J ^{[1
]}

机构：

[1] INST FOOD SCI & TECHNOL,SEATTLE,WA 98195

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 184卷 / 01期

关键词：

D O I：

10.1016/0006-291X(92)91224-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Butter clams obtained from a variety of locations along the northern British Columbia coast were assayed for the presence of individual paralytic shellfish poisoning toxins (PSPT) by HPLC and total PSPT toxicity using the mouse bioassay. Specific organs, namely the siphon, adductor muscle, foot and mantle were examined for soluble antigens that crossreacted with crab Saxitoxin-Induced Protein (SIP) using immunochemical (Western blotting) techniques. Butter clams containing high concentrations of PSPT also had several proteins that crossreacted with crab anti-SIP serum. In particular, soluble proteins with distinctly different molecular weights were found in the siphon and foot, respectively, in toxic shellfish. These proteins were absent in nontoxic butter clams. The concept of using PSPT-induced proteins in the butter clam as a screen for identifying toxic shellfish is introduced. © 1992.

引用

页码：511 / 517

页数：7

共 23 条

[1] APPEARANCE AND PARTIAL-PURIFICATION OF A HIGH MOLECULAR-WEIGHT PROTEIN IN CRABS EXPOSED TO SAXITOXIN [J].