3 TYPES OF GI-ALPHA PROTEIN OF THE GUINEA-PIG LUNG - CDNA CLONING AND ANALYSIS OF THEIR TISSUE DISTRIBUTION

被引：5

作者：

SAKANAKA, C

IZUMI, T

NAKAMURA, M

HONDA, Z

WATANABE, T

MINAMI, M

MUTOH, H

BITO, H

SEYAMA, Y

UI, M

SHIMIZU, T

机构：

[1] UNIV TOKYO,FAC PHARMACEUT SCI,DEPT PHYSIOL CHEM,TOKYO 113,JAPAN

[2] UNIV TOKYO,FAC MED,DEPT PHYSIOL CHEM,TOKYO 113,JAPAN

[3] UNIV TOKYO,FAC MED,DEPT BIOCHEM,7-3-1 HONGO,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1175卷 / 01期

关键词：

G-PROTEIN; CDNA CLONING; TISSUE DISTRIBUTION; (GUINEA PIG);

D O I：

10.1016/0167-4889(92)90009-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNA clones encoding three types of Gialpha, the alpha subunit of GTP-binding protein (Gi1alpha, Gi2alpha, and Gi3alpha), were isolated from a cDNA library of the guinea-pig lung. Nucleotide sequence analysis revealed a high degree of homology with other mammalian Gialpha cDNAs. By RNA blot analysis, the expression pattern of Gi1alpha was more tissue-specific than those of other types of Gialphas in the guinea-pig tissues examined. While Gi2alpha and Gi3alpha mRNAs were ubiquitously expressed in all tissues examined, Gi1alpha mRNA was mainly expressed in the brain, lung and kidney. These results suggest that each Gialpha protein may have a different role.

引用

页码：61 / 66

页数：6

共 49 条

[1] AN M2 MUSCARINIC RECEPTOR SUBTYPE COUPLED TO BOTH ADENYLYL CYCLASE AND PHOSPHOINOSITIDE TURNOVER [J].