DROSOPHILA AWDK-PN, A HOMOLOG OF THE METASTASIS SUPPRESSOR GENE-NM23, SUPPRESSES THE TUM-I HEMATOPOIETIC ONCOGENE

The human nm23-H1 gene is a suppressor of solid tumour metastasis in some types of cancer. It is known that nm23 genes encode nucleoside diphosphate kinase polypeptides, but the regulatory pathways involving Nm23 are unclear. One approach to understanding nm23 function is to identify loci which interact with nm23. The Drosophila awd gene, a homologue of nm23, provides a model system for this genetic analysis. We report that the dominant awd(K-pn) allele suppresses haematopoietic defects associated with the Tum-I oncogene. Premature differentiation and aggregation of Tum-I blood cells is reduced by awd(K-pn), resulting in an increased survival of Tum-I hemizygotes. Tum-I lethality is also suppressed by pn mutations, indicating the existence of a haematopoietic regulatory pathway involving the Tum-I, Awd(K-pn) and Pn proteins.