EFFECTS OF TUMOR-NECROSIS-FACTOR-ALPHA ON CONNECTIVE-TISSUE METABOLISM IN NORMAL AND SCLERODERMA FIBROBLAST-CULTURES

被引：23

作者：

TAKEDA, K ^{[1
]}

HATAMOCHI, A ^{[1
]}

ARAKAWA, M ^{[1
]}

UEKI, H ^{[1
]}

机构：

[1] KAWASAKI MED SCH, DEPT DERMATOL, KURASHIKI, OKAYAMA 70101, JAPAN

来源：

ARCHIVES OF DERMATOLOGICAL RESEARCH | 1993年 / 284卷 / 08期

关键词：

TUMOR NECROSIS FACTOR-ALPHA; NORMAL FIBROBLASTS; SCLERODERMA FIBROBLASTS; CONNECTIVE TISSUE METABOLISM; GENE EXPRESSION;

D O I：

10.1007/BF00373353

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Recent studies have demonstrated that tumor necrosis factor-alpha(TNF-alpha) selectively decreases production of collagens I and III, the major types of collagen in the dermis, and increases production of collagenase in cultured dermal fibroblasts. The effects of TNF-alpha on collagens 1, III and VI, fibronectin and collagenase gene expression by fibroblasts derived from normal individuals and patients with systemic sclerosis (SSc) were studied. SSc is characterized by excessive accumulation of collagen in the skin and in certain organs. TNF-alpha inhibited collagen production and mRNA levels of collagens I and III and of fibronectin, and stimulated collagenase activity and collagenase mRNA levels in SSs fibroblasts. Levels of mRNA for alpha1(VI) and alpha3(VI) collagen and for beta-actin were unaltered in SSc fibroblasts incubated with TNF-alpha. Similar results were observed for mRNA levels in normal fibroblasts incubated with TNF-alpha. These results suggest that TNF-alpha could be expected to be beneficial in the treatment of SSc. In addition, our results indicated that collagen-VI expression is regulated independently from expression of collagens I and III, and expression of fibronectin and collagens I and III are regulated in parallel in fibroblasts treated with TNF-alpha.

引用

页码：440 / 444

页数：5

共 33 条

[1] INDUCTION OF METALLOTHIONEIN AND OTHER MESSENGER-RNA SPECIES BY CARCINOGENS AND TUMOR PROMOTERS IN PRIMARY HUMAN-SKIN FIBROBLASTS [J].

ANGEL, P ;

POTING, A ;

MALLICK, U ;

RAHMSDORF, HJ ;

SCHORPP, M ;

HERRLICH, P .

MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (05) :1760-1766

[2] PRELIMINARY CRITERIA FOR THE CLASSIFICATION OF SYSTEMIC-SCLEROSIS (SCLERODERMA) [J].

不详 .

ARTHRITIS AND RHEUMATISM, 1980, 23 (05) :581-590

[3] INCREASED COLLAGEN-SYNTHESIS ACCOMPANYING ELEVATED MESSENGER-RNA LEVELS IN CULTURED WERNERS SYNDROME FIBROBLASTS [J].

ARAKAWA, M ;

HATAMOCHI, A ;

TAKEDA, K ;

UEKI, H .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1990, 94 (02) :187-190

[4] PENTOXIFYLLINE INHIBITS THE PROLIFERATION OF HUMAN FIBROBLASTS DERIVED FROM KELOID, SCLERODERMA AND MORPHEA SKIN AND THEIR PRODUCTION OF COLLAGEN, GLYCOSAMINOGLYCANS AND FIBRONECTIN [J].

BERMAN, B ;

DUNCAN, MR .

BRITISH JOURNAL OF DERMATOLOGY, 1990, 123 (03) :339-346

[5] HUMAN CELLULAR FIBRONECTIN - COMPARISON OF THE CARBOXYL-TERMINAL PORTION WITH RAT IDENTIFIES PRIMARY STRUCTURAL DOMAINS SEPARATED BY HYPERVARIABLE REGIONS [J].

BERNARD, MP ;

KOLBE, M ;

WEIL, D ;

CHU, ML .

BIOCHEMISTRY, 1985, 24 (11) :2698-2704

[6]

BEUTLER B, 1987, NEW ENGL J MED, V316, P379

[7] CLONING AND CHARACTERIZATION OF 5 OVERLAPPING CDNAS SPECIFIC FOR THE HUMAN PRO-ALPHA-1(I) COLLAGEN CHAIN [J].

CHU, ML ;

MYERS, JC ;

BERNARD, MP ;

DING, JF ;

RAMIREZ, F .

NUCLEIC ACIDS RESEARCH, 1982, 10 (19) :5925-5934

[8] CHARACTERIZATION OF 3 CONSTITUENT CHAINS OF COLLAGEN TYPE-VI BY PEPTIDE SEQUENCES AND CDNA CLONES [J].

CHU, ML ;

MANN, K ;

DEUTZMANN, R ;

PRIBULACONWAY, D ;

HSUCHEN, CC ;

BERNARD, MP ;

TIMPL, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 168 (02) :309-317

[9]

CZAJA MJ, 1987, J BIOL CHEM, V262, P13348

[10] CACHECTIN TUMOR NECROSIS FACTOR STIMULATES COLLAGENASE AND PROSTAGLANDIN-E2 PRODUCTION BY HUMAN SYNOVIAL-CELLS AND DERMAL FIBROBLASTS [J].

DAYER, JM ;

BEUTLER, B ;

CERAMI, A .

JOURNAL OF EXPERIMENTAL MEDICINE, 1985, 162 (06) :2163-2168

← 1 2 3 4 →