IDENTIFICATION OF ENDONUCLEOLYTIC CLEAVAGE SITES INVOLVED IN DECAY OF ESCHERICHIA-COLI TRXA MESSENGER-RNA

被引:28
作者
ARRAIANO, CM [1 ]
YANCEY, SD [1 ]
KUSHNER, SR [1 ]
机构
[1] UNIV GEORGIA,DEPT GENET,ATHENS,GA 30602
关键词
D O I
10.1128/JB.175.4.1043-1052.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The degradation of individual mRNAs in Escherichia coli has been studied through the use of a multiple mutant carrying the pnp-7 (polynucleotide phosphorylase), rnb-500 (RNase II), and rne-1 (RNase E) alleles. In this triple mutant, discrete mRNA breakdown products are stabilized in vivo at the nonpermissive temperature (Arraiano, C. M., S. D. Yancey, and S. R. Kushner, J. Bacteriol. 170:4625-4633, 1988). In the case of thioredoxin (trxA) mRNA decay, degradation fragments accumulated at early times after a shift to the nonpermissive temperature. Using Northern (RNA) blots, S1 nuclease analysis, and primer extensions, we identified a series of specific endonucleolytic cleavage sites that occur throughout the transcript in both the triple mutant and a wild-type control. The implications of the complex decay patterns observed are discussed.
引用
收藏
页码:1043 / 1052
页数:10
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