PHOTOKILLING OF MALIGNANT-CELLS WITH ULTRAFINE TIO2 POWDER

被引：195
作者：
CAI, R
HASHIMOTO, K
ITOH, K
KUBOTA, Y
FUJISHIMA, A
机构：
[1] UNIV TOKYO, DEPT SYNTHET CHEM, BUNKYO KU, TOKYO 113, JAPAN
[2] YOKOHAMA CITY UNIV, FAC MED, KANAZAWA KU, YOKOHAMA 236, JAPAN
[3] YOKOHAMA NATL UNIV, INST ENVIRONM SCI & TECHNOL, HODOGAYA KU, YOKOHAMA, KANAGAWA 240, JAPAN
来源：
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN | 1991年 / 64卷 / 04期
关键词：
D O I：
10.1246/bcsj.64.1268
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Photo-irradiated TiO2 particles drive various chemical reactions due to their strong oxidation and reduction ability. This effect is applied in order to kill malignant HeLa cells with TiO2 particles. During 10-min UV irradiation periods, HeLa cells were completely killed in the presence of TiO2 (100 mu-g cm-3). However, without irradiation these particles showed little cytotoxicity up to 360-mu-g cm-3. It is concluded that the cells were killed by hydroxyl (OH.) and perhydroxyl (HO2.) radicals produced from water by the irradiated TiO2 particles, and also that the cells were directly oxidized by photogenerated holes in TiO2. The distribution of TiO2 powders in the cells was observed using a transmission electron microscope. The TiO2 powder existed not only on the cell membrane, but also in the cytoplasm. Thus, the cells were damaged by photoexcited TiO2 not only from the cell membrane, but also from the inside of cytoplasm. Finally, the application of this malignant cell photokilling effect to photodynamic therapy is discussed.
引用
收藏
页码：1268 / 1273
页数：6
相关论文
共 21 条
[1]   PHOTOCATALYTIC HYDROGENATION OF CH3CCH WITH H2O ON SMALL-PARTICLE TIO2 - SIZE QUANTIZATION EFFECTS AND REACTION INTERMEDIATES [J].
ANPO, M ;
SHIMA, T ;
KODAMA, S ;
KUBOKAWA, Y .
JOURNAL OF PHYSICAL CHEMISTRY, 1987, 91 (16) :4305-4310
[2]   SINGLET OXYGEN GENERATION BY HEMATOPORPHYRIN-IX, UROPORPHYRIN-I AND HEMATOPORPHYRIN DERIVATIVE AT 546 NM IN PHOSPHATE BUFFER AND IN THE PRESENCE OF EGG PHOSPHATIDYLCHOLINE LIPOSOMES [J].
BLUM, A ;
GROSSWEINER, LI .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1985, 41 (01) :27-32
[3]   SUSTAINED WATER CLEAVAGE BY VISIBLE-LIGHT [J].
BORGARELLO, E ;
KIWI, J ;
PELIZZETTI, E ;
VISCA, M ;
GRATZEL, M .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1981, 103 (21) :6324-6329
[4]   HYDROGEN-PEROXIDE AND HYDROXYL FREE-RADICAL PRODUCTION BY HEMATOPORPHYRIN DERIVATIVE, ASCORBATE AND LIGHT [J].
BUETTNER, GR ;
NEED, MJ .
CANCER LETTERS, 1985, 25 (03) :297-304
[5]  
DICKSON JA, 1977, SELECTIVE HEAT SENSI, P43
[6]   PHOTOSENSITIZERS - THERAPY AND DETECTION OF MALIGNANT-TUMORS [J].
DOUGHERTY, TJ .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1987, 45 (06) :879-889
[7]   HETEROGENEOUS PHOTOCATALYTIC OXIDATION OF AROMATIC-COMPOUNDS ON TIO2 [J].
FUJIHIRA, M ;
SATOH, Y ;
OSA, T .
NATURE, 1981, 293 (5829) :206-208
[8]  
Fujishima A, 1986, PHOTOMED PHOTOBIOL, V8, P45
[9]   EFFECTS OF SCAVENGERS OF REACTIVE OXYGEN AND RADICAL SPECIES ON CELL-SURVIVAL FOLLOWING PHOTODYNAMIC TREATMENT INVITRO - COMPARISON TO IONIZING-RADIATION [J].
HENDERSON, BW ;
MILLER, AC .
RADIATION RESEARCH, 1986, 108 (02) :196-205
[10]   PHOTOELECTROCATALYTIC REDUCTION OF CARBON-DIOXIDE IN AQUEOUS SUSPENSIONS OF SEMICONDUCTOR POWDERS [J].
INOUE, T ;
FUJISHIMA, A ;
KONISHI, S ;
HONDA, K .
NATURE, 1979, 277 (5698) :637-638
123