PURIFICATION OF PROCOLLAGEN TYPE-II BY COVALENT CHROMATOGRAPHY WITH ACTIVATED THIOL-SEPHAROSE 4B

被引：15
作者：
ANGERMANN, K
BARRACH, HJ
机构：
[1] Institut für Toxikologie, Embryonal-Pharmakologie der Freien Universität Berlin, 1000 Berlin 33
来源：
ANALYTICAL BIOCHEMISTRY | 1979年 / 94卷 / 02期
关键词：
D O I：
10.1016/0003-2697(79)90356-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Procollagen type II was isolated from Swarm chondrosarcoma of rat by NaCl-extraction and DEAE-cellulose chromatography and further purified from remaining type II collagen using activated thiol-Sepharose 4B for covalent chromatography. The purity of the separated collagen fractions was tested by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The results demonstrate that this method is a useful tool for separating procollagen from other collagenous material, which does not contain thiol groups. © 1979.
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页码：253 / 258
页数：6
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