ENHANCED SYNTHESIS OF CYSTEINYL LEUKOTRIENES IN ATOPIC-DERMATITIS

被引：33

作者：

FAULER, J ^{[1
]}

NEUMANN, C ^{[1
]}

TSIKAS, D ^{[1
]}

FROLICH, JC ^{[1
]}

机构：

[1] HANNOVER MED SCH,DEPT DERMATOL,HANNOVER,GERMANY

来源：

BRITISH JOURNAL OF DERMATOLOGY | 1993年 / 128卷 / 06期

关键词：

D O I：

10.1111/j.1365-2133.1993.tb00256.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Synthesis of cysteinyl leukotrienes was assessed in patients with atopic dermatitis (AD; n = 8) and healthy volunteers (n = 8) by measuring urinary excretion of leukotriene E4 (LTE4), the main index metabolite of cysteinyl leukotrienes in man. Using this non-invasive method we demonstrated a significant (P<0.05) 4.5-fold increase in excretion of LTE4 compared with healthy volunteers. The identity of LTE4 was unequivocally demonstrated by gas chromatography-mass spectrometry/mass spectrometry (GC-MS/MS). LTE4 was routinely measured by radioimmunoassay (RIA), and quantitative measurement of LTE4 by RIA was validated by GC-MS/MS. There was a linear correlation between LTE4 measured by RIA and by GC-MS/MS (r = 0.994). In representative samples, LTE4 was also quantitatively assessed by GC-MS/MS. In these samples, LTE4 values obtained by GC-MS/MS differed <10% from those obtained by RIA. The present findings suggest that cysteinyl leukotrienes play a role in AD.

引用

页码：627 / 630

页数：4

共 15 条

[1] EFFECT OF DIETARY SUPPLEMENTATION WITH N-3 FATTY-ACIDS ON CLINICAL MANIFESTATIONS OF PSORIASIS [J].