A MONOCLONAL-ANTIBODY WHICH BINDS A CARBOHYDRATE MOIETY BROADLY EXPRESSED ON RAINBOW-TROUT LEUKOCYTES

被引：20
作者：
GREENLEE, AR [1 ]
RISTOW, SS [1 ]
机构：
[1] WASHINGTON STATE UNIV,DEPT ANIM SCI,PULLMAN,WA 99164
来源：
FISH & SHELLFISH IMMUNOLOGY | 1993年 / 3卷 / 05期
关键词：
MONOCLONAL ANTIBODY; CARBOHYDRATE; FISH; TROUT; LEUKOCYTE;
D O I：
10.1006/fsim.1993.1032
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
A monoclonal antibody, anti-2PBL4X (IgG1), was developed which induces aggregation of trout leucocytes and suppresses in vitro mixed lymphocyte cultures and cytolysis by nonspecific cytotoxic cells. Cellular aggregation and suppression of functional assays is mediated by microgram quantities of the antibody and is dose-dependent. The epitope recognised by anti-2PBL4X is present on approximately 75% of trout peripheral blood cells, 84% of trout anterior kidney cells and 81% of trout thymocytes, as determined by flow cytometry. Western blot analysis of an extract of trout leucocytes reveals that multiple bands are stained by anti-2PBL4X. Digestion of the extract with the endoglycosidase F/N glycosidase-F from Flavobacterium meningosepticum abolishes the staining pattern, indicating that the antibody recognises a broadly expressed N-linked oligosaccharide on trout leucocytes. © 1993 Academic Press. All rights reserved.
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页码：317 / 330
页数：14
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  • [1] Ainsworth A.J., Dexiang C., Greenway T., Characterization of monoclonal antibodies to channel catfish (Ictalurus punctatus Rafinesque) leucocytes, Veterinary Immunology and Immunopathology, 26, pp. 81-92, (1990)
  • [2] Bierer B.E., Sleckman B.P., Ratnofsky S.E., Burakoff S.J., The biologic roles of CD2, CD4, and CD8 in T cell activation, Annual Review of Immunology, 7, pp. 579-599, (1989)
  • [3] Blazer V.S., Bennett R.O., Wolke R.E., The cellular immune response of rainbow trout (Salmo gairdneri Richardson) to sheep red blood cells, Developmental and Comparative Immunology, 8, pp. 81-87, (1984)
  • [4] Bly J.E., Miller N.W., Clem L.W., A monoclonal antibody for neutrophils in normal and stressed catfish, Developmental and Comparative Immunology, 14, pp. 211-221, (1990)
  • [5] Butcher E.C., Rouse R.V., Coffman R.L., Nottenburg C.N., Hardy R.R., Weissman I.L., Surface phenotype of Peyer’s patch germinal center cells: Implication for the role of germinal centers in B cell differentiation, Journal of Immunology, 129, pp. 2698-2707, (1982)
  • [6] Chambers W.H., Vujanovic N.L., Deleo A.B., Olszowy M.W., Herberman R.B., Hiserodt J.C., Monoclonal antibody to a triggering structure expressed on rat natural killer cells and adherent lymphokine activated killer cells, Journal of Experimental Medicine, 169, pp. 1373-1389, (1986)
  • [7] Deluca D., Wilson M., Warr G.W., Lymphocyte heterogeneity in the trout, Salmogairdneri, defined with monoclonal antibodies to Ig M, European Journal of Immunology, 13, pp. 546-551, (1983)
  • [8] Egberts E., Secombes C.J., Wellink J.E., Van Groningen J.M., Van Muiswinkel W.B., Analysis of lymphocyte heterogeneity in carp Cyprinus carpio L. Using monoclonal antibodies, Developmental and Comparative Immunology, 7, pp. 749-754, (1983)
  • [9] Elder J.H., Alexander S., Endo-β-N acetylglucoaminidase F:N glycosidase from Flavobacterium meningosepticum that cleaves both high-mannose and complex glycoproteins, Proceedings of the National Academy of Sciences U.S.A, 79, pp. 4540-4544, (1982)
  • [10] Etlinger H.M., Hodgkins H.O., Chiller J.M., Evolution of the lymphoid system. II. Evidence for immunoglobulin determinants on all rainbow trout lymphocytes and demonstration of mixed lymphocyte reaction, European Journal of Immunology, 7, pp. 881-887, (1977)
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