AGROBACTERIUM-MEDIATED TRANSFORMATION OF CITRUS STEM SEGMENTS AND REGENERATION OF TRANSGENIC PLANTS

被引：136
|
作者：
MOORE, GA
JACONO, CC
NEIDIGH, JL
LAWRENCE, SD
CLINE, K
机构：
[1] Fruit Crops Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, 32611, FL
来源：
PLANT CELL REPORTS | 1992年 / 11卷 / 5-6期
关键词：
CARRIZO CITRANGE; KEY LIME (C-AURANTIFOLIA); GENETIC TRANSFORMATION;
D O I：
10.1007/BF00235073
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
A method for Agrobacterium-mediated transformation of Citrus and organogenic regeneration of transgenic plants is reported. Internodal stem segments were co-cultured with Agrobacterium harboring binary vectors that contained the genes for the scorable marker beta-glucuronidase (GUS) and the selectable marker NPT-II. A low but significant percentage (less-than-or-equal-to 5%) of the shoots regenerated in the presence of 100-mu-g/ml kanamycin were GUS+. Polymerase chain reaction (PCR) analysis confirmed that GUS+ shoots contained T-DNA. Two plants established in soil were shown to be transgenic by Southern analysis.
引用
收藏
页码：238 / 242
页数：5
相关论文
共 50 条
  • [31] Efficient production of transgenic tomatoes via Agrobacterium-mediated transformation
    Kaur, P.
    Bansal, K. C.
    BIOLOGIA PLANTARUM, 2010, 54 (02) : 344 - 348
  • [32] Agrobacterium-Mediated Transformation of Embryogenic Calli of Anliucheng and Regeneration of Plants Containing the Chimaeric Ribonuclease Gene
    LI Dong-dong
    Agricultural Sciences in China, 2003, (02) : 13 - 17
  • [33] In vitro regeneration and Agrobacterium-mediated genetic transformation of Euonymus alatus
    Chen, Yongqin
    Lu, Litang
    Deng, Wei
    Yang, Xingyu
    McAvoy, Richard
    Zhao, Degang
    Pei, Yan
    Luo, Keming
    Duan, Hui
    Smith, William
    Thammina, Chandra
    Zheng, Xuelian
    Ellis, Donna
    Li, Yi
    PLANT CELL REPORTS, 2006, 25 (10) : 1043 - 1051
  • [34] In vitro regeneration and Agrobacterium-mediated genetic transformation of Euonymus alatus
    Yongqin Chen
    Litang Lu
    Wei Deng
    Xingyu Yang
    Richard McAvoy
    Degang Zhao
    Yan Pei
    Keming Luo
    Hui Duan
    William Smith
    Chandra Thammina
    Xuelian Zheng
    Donna Ellis
    Yi Li
    Plant Cell Reports, 2006, 25 : 1043 - 1051
  • [35] Agrobacterium-mediated genetic transformation of Nagpur mandarin (Citrus reticulata Blanco)
    Khawale, R. N.
    Singh, S. K.
    Garg, G.
    Baranwal, V. K.
    Ajirlo, S. Alizadeh
    CURRENT SCIENCE, 2006, 91 (12): : 1700 - 1705
  • [36] Agrobacterium-mediated transformation of plants: Basic principles and influencing factors
    Alimohammadi, Mohammad
    Bagherieh-Najjar, Mohammad B.
    AFRICAN JOURNAL OF BIOTECHNOLOGY, 2009, 8 (20): : 5142 - 5148
  • [37] An evaluation of factors affecting the efficiency of Agrobacterium-mediated transformation of Citrus paradisi (Macf.) and production of transgenic plants containing carotenoid biosynthetic genes
    Costa, MGC
    Otoni, WC
    Moore, GA
    PLANT CELL REPORTS, 2002, 21 (04) : 365 - 373
  • [38] Agrobacterium-Mediated Transformation for the Development of Transgenic Crops; Present and Future Prospects
    Rahman, Saleem Ur
    Khan, Muhammad Omar
    Ullah, Rahim
    Ahmad, Fayaz
    Raza, Ghulam
    MOLECULAR BIOTECHNOLOGY, 2024, 66 (08) : 1836 - 1852
  • [39] Transgenic sugar beet tolerant to imidazolinone obtained by Agrobacterium-mediated transformation
    E. M. Kishchenko
    I. K. Komarnitskii
    N. V. Kuchuk
    Cytology and Genetics, 2011, 45 : 148 - 152
  • [40] Agrobacterium-mediated transformation of embryogenic calluses of Ponkan mandarin and the regeneration of plants containing the chimeric ribonuclease gene
    Li, DD
    Shi, W
    Deng, XX
    PLANT CELL REPORTS, 2002, 21 (02) : 153 - 156
12345